Rapid identification of constituents of Urtica hyperborea using UPLC-ESI-Q-TOF-MS/MS method.
10.19540/j.cnki.cjcmm.20190118.005
- Author:
Ri-Na SU
1
;
Wei-Zao LUO
2
;
Rong-Rui WEI
1
;
Wu-Li-Ji AO
3
;
Guo-Yue ZHONG
1
Author Information
1. Research Center for Traditional Chinese Medicine Research and Ethnic Minority Medicine,Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China.
2. Chongqing Academy of Chinese Meteria Medica Chongqing 400065,China.
3. School of Mongol Medicine,Inner Mongolia University for Nationalities Tongliao 028000,China.
- Publication Type:Journal Article
- Keywords:
UPLC-ESI-Q-TOF-MS/MS;
Urtica hyperborea;
chemical composition;
rapid identification
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Flavonoids;
Lignans;
Phenols;
Phytochemicals;
analysis;
Plant Extracts;
analysis;
Tandem Mass Spectrometry;
Urticaceae;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(8):1607-1614
- CountryChina
- Language:Chinese
-
Abstract:
This paper deals with the application of ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS/MS) method to rapidly determine and analyze the chemical constituents of methanol extract of Urtica hyperborea. We employed UPLC YMC-Triart C18(2. 1 mm×100 mm,1. 9 μm) column to UPLC analysis with acetonitrile-water(containing 0. 4% formic acid) in gradient as mobile phase. The flow rate was 0. 3 m L·min-1 gradient elution and column temperature was 30℃; the injection volume was 4 μL. ESI ion source was used to ensure the data collected in anegative ion mode. The chemical components of U. hyperborea were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data.The results indicated that a total of 31 compounds were identified,including 8 flavonoids,14 phenolic compounds,8 phenylpropanoids(4 coumarins and 4 lignans),and 1 steroidal compound,13 of which were confirmed by comparison. The UPLC-ESI-Q-TOF-MS/MS method could rapid identify the chemical components of U. hyperborea. The above compounds were discovered in U. hyperborea for the first time,which could provide theoretical foundation for further research on the basis of the pharmacodynamics of U. hyperborea.
