Evaluation of Material Permeability of Type I Collagen Hydrogel.
10.3969/j.issn.1671-7104.2018.02.017
- Author:
Xiaojie DUAN
1
;
Shan LIU
2
;
Yue MA
3
;
Xueliang SUN
2
;
Jinheng WANG
3
;
Anliang SHAO
1
;
Liming XU
1
Author Information
1. National Institutes for Food and Drug Control, Beijing, 102629.
2. Arthro-Anda Tianjin Biologic Technology Co., Ltd. Tianjin, 300308.
3. Beijing Sanyao Science & Technology Development Co., Beijing, 100176.
- Publication Type:Journal Article
- Keywords:
BSA-FITC;
hydrogel;
indicator;
permeability;
permeability media;
transparent rate
- MeSH:
Collagen Type I;
chemistry;
Hydrogels;
chemistry;
Materials Testing;
Permeability
- From:
Chinese Journal of Medical Instrumentation
2018;42(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish an experimental method for evaluating material permeability of type I collagen hydrogels.
METHODS:Using BSA-FITC as an indicator, by combining BSA-FITC with PBS they were used as permeability media, and using transwell load hydrogen sample to detect BSA-FITC transparent rate.
RESULTS:In the concentration range of 100 μg·mL~0.781 μg·mL, the standard curve ≥ 0.99, Lower Limit of Quantity (LLOQ) is 3.125 μg·mL, RSD <5%, detection recovery rate is in the range of 80%~120%.
CONCLUSIONS:In this study, we established an experimental method for evaluating material permeability of hydrogel. The BSA-FITC transparent rate of type I collagen hydrogel was 100% at 28 h.