Changes of DC Subsets and CD80 and CD86 Expression in Peri-pheral Blood of Patients with ITP and Their Correlation with Efficacy of Dexamethasone Treatment.
10.7534/j.issn.1009-2137.2018.06.029
- Author:
Xiao-Juan LI
1
;
Xiao-Shuang SONG
2
Author Information
1. Department of Oncology & Hematology, Jinan Third People's Hospital, Jinan 250132, Shandong Province, China.E-mail:greenmount@126.com.
2. Department of Oncology & Hematology, Jinan Third People's Hospital, Jinan 250132, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
B7-1 Antigen;
Dendritic Cells;
Dexamethasone;
Humans;
Inosine Triphosphate;
Retrospective Studies
- From:
Journal of Experimental Hematology
2018;26(6):1752-1756
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the changes of DC subsets and the expression of CD80 and CD86 in peripheral blood of ITP patients and their correlation with dexamethasone efficacy.
METHODS:Peripheral blood sample of 80 cases of ITP and 20 normal controls from June 2015 to June 2017 in our hospital were retrospectively analyzed. The specific distribution of DC subsets in the peripheral blood of all the subjects was detected by flow cytometry, and the expressions of CD80 and CD86 were detected by ELISA.
RESULTS:The proportion of DC2 in DC subsets of ITP patients before treatment was significantly higher than that in normal control group (P<0.05). The proportion of DC2 in DC subset of ITP patients was still significantly higher than that of the control group (P<0.05). The level of CD80 expression on DC1 and DC2 in ITP patients before treatment was significantly higher than that in the normal control group (P<0.05), and the expression level of CD86 on DC2 was significantly higher than that of the normal control group (P<0.05). Both IL-2 and IFN- γ levels in the patients before the treatment were significantly higher than those in the normal control group (P<0.05), and the expression levels after treatment with dexamethasone decreased significantly. Before treatment, both IL-4 and IL-10 levels in ITP patients were significantly lower than those in the normal control group (P<0.05), and their expression levels after treatment with dexamethasone significantly increased (P<0.05).
CONCLUSION:The incidence of ITP patients closely relates with the level and dysfunction of DC subsets in peripheral blood and the expression levels of IL-2, IL-4, IL-10, IFN- γ, which significantly correlates with the efficacy of dexamethasone.
