Effect of Notch Signaling Pathway on Differentiation of Mouse Embryonic Stem Cells into Hematopoietic Stem Cells or Hematopoietic Progenitor Cells by VEGF.
10.7534/j.issn.1009-2137.2018.06.033
- Author:
Bo SONG
1
;
Ri-Ling CHEN
2
;
Feng-Ling LIAO
3
Author Information
1. Department of Pediatrics, Guangdong Medical University,Guangdong Province, China.
2. Medical center of Pediatrics, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China.E-mail: chenrl319@163.com.
3. Medical center of Pediatrics, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
Hematopoietic Stem Cells;
Mice;
Mouse Embryonic Stem Cells;
Receptors, Notch;
Signal Transduction;
Vascular Endothelial Growth Factor A
- From:
Journal of Experimental Hematology
2018;26(6):1780-1784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of Notch signaling pathways on differentiation of mouse embryonic stem cells(ESC) into haematopoietic stem cells or haematopoietic progenitors cells(HSC/HPC).
METHODS:Mouse embryonic stem cells were proliferated in vitro to form embryoid bodies; the differentiation of embryoid bodies should be induced in vitro, the experiments were divided into BE, control, VEGF, DAPT and VEGF-DAPT groups; HSC/HPC ohenotype: CD117D34Sca1 was detected by flow cytometry; the related gene expression was detected by RT-PCR.
RESULTS:The number of VEGF-induced HSC/HPC in VEGF group was significantey higher than that in the control and EB group (P<0.05), suggesting that VEGF promotes ESC differentiation to HSC/HPC; the number of DAPT-induced HSC/HPC in DAPT group was significanty higher than that in the Control and EB groups(P<0.05), suggesting that DAPT promotes ESC differentiation to HSC/HPC; the number of VEGF+DAPT-induced HSC/HPC in VEGF-DAPT group was significantly higher than that in VEGF and DAPT groups(P<0.05), suggesting that DAPT and VEGF play a synergistic role to promote differentiation of ESC into HSC/HPC.
CONCLUSION:Notch signal pathway inhibits differentiation of ESC into HSC / HPC by VEGF.
