Expression of Ikaros and FUT4 in Children's Acute Lymphoblastic Leukemia and Their Relationship.
10.7534/j.issn.1009-2137.2019.01.001
- Author:
Li-Jun YI
1
;
Hong LI
2
;
Zhi-Bing GUO
2
;
Zhi-Qiang LIU
2
;
Jing ZHOU
2
;
Chong-Jun WU
2
;
Xiao-Ping ZENG
3
Author Information
1. Medical School of Nanchang University,Nanchang 330006,Jiangxi Province,China.Jiangxi Provincial Children's Hospital,Nanchang 330006,Jiangxi Province,China.
2. Jiangxi Provincial Children's Hospital,Nanchang 330006,Jiangxi Province,China.
3. Medical School of Nanchang University,Nanchang 330006,Jiangxi Province,China.E-mail：3092931971@qq.com.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Child;
Fucosyltransferases;
metabolism;
Humans;
Ikaros Transcription Factor;
metabolism;
Lewis X Antigen;
metabolism;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
Protein Isoforms;
Recurrence
- From:
Journal of Experimental Hematology
2019;27(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the possible molecular mechanism of Ikaros regulation on FUT4 expression by analyzing the correlation of the functional state of Ikaros with level of FUT4 expression, so as to provide the theoretical basis for personalized treatment in children with ALL.
METHODS:The subtypes of Ikaros were identified by nested PCR and sequencing. The expression level of FUT4 was detected by quantitative PCR and analyzed by ΔΔCt method in the early stage of treatment, remission and relapse of ALL.
RESULTS:Ik1 and Ik2 were the main functional subtypes, and the dominant negative Ikaros was Ik6; the Ik6 was detected in 23 patients with ALL. It was found that 2.73% patients expressing Ik6 alone and 18.18% patients with heterozygous expression were detected. The expression of FUT4 in the newly diagnosed ALL was higher than that in the control group, and the functional Ikaros negatively correlated with the FUT4 expression(r=-0.6329).
CONCLUSION:Dominant negative Ikaros closely correlated with the relapse of acute lymphoblastic leukemia in children. The functional Ikaros negatively correlated with FUT4 expression. Ikaros inhibit the transcriptional activity of FUT4, that may be the molecular mechanism of Ikaros regulating the expression of FUT4.
