Expression and Function of miR-99a-5p in Bone Marrow of Patients with MDS.
10.7534/j.issn.1009-2137.2019.01.022
- Author:
Ying LE
1
;
Xu-Pai ZHANG
2
;
Yan-Qiu XIONG
1
;
Hong-Ying LI
3
;
Wei-Hua ZHAO
1
;
Yuan LONG
1
;
Jun LUO
1
;
Peng CHENG
1
;
Zhen-Fang LIU
4
Author Information
1. Department of Hematology, The First Affliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
2. Department of Hematology, The First People's Hospital of Neijiang, Neijiang 641000, Sichuan Province, China.
3. Department of Hematology, The First People's Hospital of Nanning, Nanning 530021,Guangxi Zhuang Autonomous Region,, China.
4. Department of Hematology, The First Affliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.E-mail: Liuliuzhenfang@126.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow;
Computational Biology;
Humans;
Karyotyping;
MicroRNAs;
Myelodysplastic Syndromes
- From:
Journal of Experimental Hematology
2019;27(1):134-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression of miR-99a-5p in myelodysplastic syndrome (MDS), to predict the target genes and to analyze its function by using bioinformatics.
METHODS:The expression levels of bone marrow miR-99a-5p in MDS patients were detected by qRT-PCR, and the correlation of miR-99a-5p expression with clinical pathological characteristics, percentage of marrow blasts , chromosome karyotype and peripheral blood hemogram were analyzed. The target genes of miR-99a-5p were predicted by Targetscan, Miranda and Microcosm, and the intersection of the predicted results of 3 softwares was used as a potential target gene for miR-99a-5p.
RESULTS:The expression level of bone marrow miR-99a-5p in MDS patients was significantly higher than that of healthy controls (P<0.01); According to the prognostic score of IPSS, MDS patients were divided into relatively low risk group (including low risk group and intermediate risk group 1) and relatively high risk group (including intermediate risk group 2 and high risk group). Analysis showed that the expression level of bone marrow miR-99a-5p in relatively low risk group was 2.40 times higher than that in healthy control group (P<0.01), the expression level of bone marrow miR-99a-5p in relatively high risk group was 6.66 times higher than that in healthy control group (P<0.01), the expression level of miR-99a-5p in relatively high risk group was 2.80 times higher than that in the relatively low risk group ( P<0.01) ; the expression level of bone marrow miR-99a-5p in t-MDS group was 6.35 times higher than that in healthy control group (P<0.001). There was a significant positive correlation between the expression level of miR-99a-5p and the percentage of marrow blasts (P<0.01), but the expression of miR-99a-5p did not correlate with chromosome karyotype and peripheral blood hemogram; In this study it was obtained that ST5 might participate in pathological mechanism of MDS by bioinformatic analyses and references.
CONCLUSION:The expression levels of miR-99a-5p is up-regulated in MDS patients, and its expression showed a rising tendency which may be involved in the pathogenesis of MDS by regulating target gene ST5.
