Level of Regulatory B Cells in Patients with Immune Thrombocytopenia and Its Clinical Significance.
10.7534/j.issn.1009-2137.2019.01.028
- Author:
Shi-Rong ZHU
1
;
Hai-Yan CHEN
2
;
Ming-Jing WANG
3
;
Yong-Gang XU
4
;
Ri-Cheng QUAN
4
;
Xiao-Qing DING
2
;
Pan ZHAO
3
;
Hong-Zhi WANG
4
;
Xiao-Qing GUO
4
;
Xiao-Mei HU
5
Author Information
1. Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Sciences, Beijing, 100091, China.Graduate School, Beijing University of Traditional Chinese Medicine, Beijing, 100029, China.
2. Department of Hematology, Dongfang Hospital, Beijing University of Traditional Chinese Medicine,Beijing, 100078, China.
3. Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Sciences, Beijing, 100091, China.Graduate School, China Academy of Traditional Chinese Medical Sciences, Beijing, 100700, China.
4. Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Sciences, Beijing, 100091, China.
5. Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Sciences, Beijing, 100091, China.E-mail: huxiaomei_2@163.com.
- Publication Type:Journal Article
- MeSH:
B-Lymphocytes, Regulatory;
Humans;
T-Lymphocytes, Regulatory;
Th17 Cells;
Thrombocytopenia
- From:
Journal of Experimental Hematology
2019;27(1):175-179
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of regulatory B cells (Breg) in pathogenesis of immune thrombocytopenia(ITP) and its clinical significance.
METHODS:A total of 40 ITP patients and 20 normal controls were enrolled in this study. The content of Breg, Th1, Th2, Th17 and Treg cells were detected by flow cytometry (FCM). The expression level of IL-10,TGF-β, CD40 and CD40L was detected by AimPlex Flow High Throughput Screening Technology.
RESULTS:The of Breg cells in ITP patients was significantly lower than that in normal controls (P<0.05)，the expression levels of IL-10,TGF-β and CD40L in ITP patients were also significantly lower than those in normal controls (P<0.05). The contents of Th1 cells in ITP patients were significantly higher than that in normal controls (P<0.05), whereas the contents of Th2, Th17 and Treg cells in ITP patients were significantly lower than those in normal controls (P<0.05).
CONCLUSION:The Breg cells may play an important role in the pathogenesis of ITP.
