Regulatory Effect of Exosomes Derived from Human Umbiilcal Cord Mesenchymal Stem Cells on Treg and TH17 Cells.
10.7534/j.issn.1009-2137.2019.01.036
- Author:
Li-Yan GUO
1
,
2
;
Pei-Long LAI
3
;
Su-Xia GENG
3
;
Xiao-Mei CHEN
3
;
Yu-Lian WANG
3
;
Tian HUANG
3
;
Xin DU
3
;
Jian-Yu WENG
1
,
4
Author Information
1. School of Medicine, South China University of Technology
2. Guangzhou 510006, Guangdong Province, China.Department of Hematology, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangdong Elderly Research Institute, Guangzhou 510080, Guangdong Province, China.
3. Department of Hematology, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangdong Elderly Research Institute, Guangzhou 510080, Guangdong Province, China.
4. Guangzhou 510006, Guangdong Province, China.Department of Hematology, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangdong Elderly Research Institute, Guangzhou 510080, Guangdong Province, China.E-mail: wengjianyu1969@163.com.
- Publication Type:Journal Article
- MeSH:
Exosomes;
Humans;
Leukocytes, Mononuclear;
Mesenchymal Stem Cells;
T-Lymphocytes, Regulatory;
Th17 Cells;
Umbilical Cord
- From:
Journal of Experimental Hematology
2019;27(1):221-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of exosomes from human umbilical cord mesenchymal stem cells on the development of Treg and TH17 cells.
METHODS:Exosomes from the serum-free-culture supernatants of hUC-MSC were harvested by ultracentrifugation. The electron microscopy, nanoparticle tracking analysis and western blot were used to identify the hUC-MSC-exosomes, such as the morphology, the paticle chameter, and the protein content. The PBMC stimulated with anti-CD3/CD28 were incubated with the exosomes for five days, and then the percentage changes of Treg and TH17 cells were analyzed by using flow cytometry.
RESULTS:The hUC MSC-derived exosomes were saucer-like in morphology the averge diameter was approximately 142 nm. They were identified as positive for CD9 and CD63. Flow cytometry showed that the proportion of CD4CD25Foxp3 Treg cells in the PBMC were significantly higher, but the proportion of CD4IL17A T cells in the hUC-MSC-exosome group was obviously lower than that in the group without the hUC-MSC-exosom (control group) (P<0.05).
CONCLUSION:The hUC-MSC-exosomes have an immunomodulatory effect on T cells in vitro by increasing the ratio of Treg and reducing the ratio of TH17 cells, expecting the hUC-MSC-exosom as a novel cell-free target for immunotherapy.
