Detection and Analysis of T Lymphocyte Subsets and B Lymphocytes in Patients with Acute Leukemia.
10.19746/j.cnki.issn.1009-2137.2019.02.003
- Author:
Juan CHENG
1
;
Hai-Zhen MA
2
;
Hao ZHANG
2
Author Information
1. Department of Hematology, The First Hospital of Lanzhou University, Laznhou 730000, Gansu Province, China,E-mail: chenggu029@163.com.
2. Department of Hematology, The First Hospital of Lanzhou University, Laznhou 730000, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
B-Lymphocytes;
Flow Cytometry;
Humans;
Killer Cells, Natural;
Leukemia, Myeloid, Acute;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
T-Lymphocyte Subsets
- From:
Journal of Experimental Hematology
2019;27(2):327-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of T lymphocyte subsets, B lymphocyte and NK cells in peripheral blood of patients with acute leukemia at different periods and their significance.
METHODS:The peripheral T lymphocyte subsets and B lymphocyte of 95 patients with acute leukemia [(43 cases of acute lymphoblastic leukemia (ALL), 52 cases of acute myeloid leukemia (AML)] and 50 normal people were detected by flow cytometry respectively.
RESULTS:The positive rate of CD3, CD3CD4, CD3CD8, NK cells and CD4/CD8 in the patients with newly diagnosed acute leukemia were significantly lower than those in normal controls (P<0.05) , but increased obviously after complete remission. The positive rate of Treg cells in the patients with newly diagnosed leukemia group was significantly higher than that in normal controls (P<0.01) , but decreased obviously after complete remission. Positive rate of CD3CD19 cells in the patients with newly diagnosed acute myeloid leukemia was significantly lower , but higher in the patients with newly diagnosed acute lymphoblastic leukemia than that in normal controls with statistical significant difference (P<0.05) , and increased obviously in the patients with acute myeloid leukemia (P<0.05) after complete remission , but decreased in the patients with acute lymphoblastic leukemia (P<0.01) after complete remission or no-remission.
CONCLUSION:Changes of T lymphocyte subsets, B lymphocytes and NK cells in the patients with newly diagnosed acute leukemia are significant, thus the detection of T lymphocyte cell subsets, B lymphocytes and NK cells can provide some evidences. for evaluation of the disease severity, curative efficiency and prognosis of patients with acute leukemia.
