Expression and Clinical Significance of PD-L1 and MicroRNA-138-5p in Patients with Acute Myeloid Leukemia.
10.19746/j.cnki.issn.1009-2137.2019.02.010
- Author:
Chun-Yan HUANG
1
;
Xian-Feng ZHA
1
;
Wang-Rong WEN
2
Author Information
1. Center of Clinical Laboratorial Medicine，The First Affiliated Hospital of Jinan University，Guangzhou 510630, Guangdong Province, China.
2. Center of Clinical Laboratorial Medicine，The First Affiliated Hospital of Jinan University，Guangzhou 510630, Guangdong Province, China,E-mail: wenwangrong@yeah.net.
- Publication Type:Journal Article
- MeSH:
B7-H1 Antigen;
genetics;
Humans;
Leukemia, Myeloid, Acute;
genetics;
Leukocytes, Mononuclear;
MicroRNAs;
genetics;
Remission Induction
- From:
Journal of Experimental Hematology
2019;27(2):373-378
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and clinical significance of PD-L1 and microRNA-138-5p in the peripheral blood mononuclear cells of patients with acute myeloid leukemia.
METHODS:The SYBR GreenⅠreal-time PCR was used to detect the expression levels of PD-L1 mRNA and miR-138 in 20 cases of primary AML, 9 cases of relapsed/refractory AML and 8 cases of complete remission. The samples of peripheral blood of 20 healthy peoples were used as controls.
RESULTS:The expression levels of PD-L1 in both the primary AML and the relapsed/refractory AML groups were significantly higher than those in the healthy control group (P<0.01), and the expression level of PD-L1 in the relapsed/refractory AML group was significantly higher than that in the primary AML group (P<0.01). The expression level of miR-138 in both the primary AML and the relapsed/refractory AML groups were significantly lower than that in the healthy control group (P<0.01). The 8 sampes out of 20 cases of primary AML patients achieved complete remission (CR) were collected and detected. The results showed that the expression level of miR-138 in the complete remission group was higher than that in the primary AML group (P<0.05), but the expression level of PD-L1 mRNA in the CR group was not significantly different from that in the primary AML group (P>0.05). and there was a negative correlation between PD-L1 mRNA and miR-138 in primary AML patients (P<0.05).
CONCLUSION:The expression of PD-L1 increases and the expression of miR-138 down-regulates in PBMNCs of AML patients, furthermore, both correlate each other.
