Exploration of the Role of Tumor Suppressor Genes Foxo1 and PTEN in the Tumorigenesis of Mouse Natural Killer-Cell Lymphoma.
10.19746/j.cnki.issn.1009-2137.2019.02.021
- Author:
Yan JIANG
1
,
2
;
Hui-Yang LIAO
3
;
Qiu-Shi YANG
4
;
Yang CHEN
5
;
Ya-Ning HU
5
;
Shun-Zong YUAN
6
;
Su Hang SU
1
,
7
Author Information
1. Clinical College of 307 Hospital, PLA, Anhui Medical University
2. Hefei 230032, Anhui Province, China,Department of Lymphoma, General Hospital of PLA Fifth Medical Center, Beijing 100071, China.
3. Laboratory of Oncology, General Hospital of PLA Fifth Medical Center, Beijing 100071, China.
4. Department of Lymphoma, General Hospital of PLA Fifth Medical Center, Beijing 100071, China.
5. Department of Clinical Laboratorial Examination, General Hospital of PLA Fifth Medical Center, Beijing 100071, China.
6. Department of Clinical Laboratorial Examination, General Hospital of PLA Fifth Medical Center, Beijing 100071, China,E-mail: yuanshunzong@126.com.
7. Hefei 230032, Anhui Province, China,Department of Lymphoma, General Hospital of PLA Fifth Medical Center, Beijing 100071, China,E-mail:suhang307@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Transformation, Neoplastic;
Forkhead Box Protein O1;
Genes, Tumor Suppressor;
Killer Cells, Natural;
Lymphoma;
Mice;
Mice, Knockout
- From:
Journal of Experimental Hematology
2019;27(2):439-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore whether tumor suppressor gene Foxo1 and PTEN play a critical role in the tumorigenesis of mouse natural killer-cell lymphoma.
METHODS:NKp46-iCre mice were crossed with mice carrying floxed Foxo1 alleles (Foxo1) as well as floxed PTEN alleles (PTEN) to generate mice in which Foxo1 and PTEN in NK cells were knock-out, referred as Foxo1PTEN. The growth and development of the mice and tumor formation were observed. The flow cytometry was used to detect the percentages of NK cells in main lymphatic organs. B16F10 metanoma model of tumor metastasis was utilized to investigate NK cell-mediated tumor surveillance in vivo after NK cells special deletion of Foxol and PTEN.
RESULTS:The mouse model with NK cell-special Foxo1 and PTEN double knockout was established. Compared with control group (Foxo1PTEN mice), Foxo1PTEN mice were born alive and appeared to be healthy over a period of 46 weeks. No spontaneous tumor formation was observed at this stage. There were no significant differences in NK cell percentages of gated lymphocytes from various organs including blood, bone marrow, peripheral lymph node and spleen between Foxo1PTEN mice and Foxo1PTEN mice [PB: 4.76%±0.46% vs 4.17%±0.64% (P＞0.05, n=8); BM: 1.13%±0.23% vs 1.31%±0.10% (P＞0.05, n=8) ; LN: 0.50%±0.10% vs 0.85%±0.20% (P＞0.05, n=8); SP: 4.41%±0.65% vs 3.50%±0.24% (P＞0.05, n=8)]. B16F10 melanoma metastasis model of tumor was established, No differences in median survival time were observed in the 2 types of mice (P＞0.05, n=13).
CONCLUSION:The simultaneous deletion of the Foxo1 and PTEN genes may not plays significant role in the tumorigenesis of mouse natural killer-cell lymphoma and NK cell-mediated tumor surveillance in vivo.
