Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta.
10.1016/j.joim.2019.01.006
- Author:
Asmae ASSAIDI
1
;
Ikram DIB
1
;
Monique TITS
2
;
Luc ANGENOT
2
;
Said BELLAHCEN
1
;
Nourelhouda BOUANANI
1
;
Abdelkhaleq LEGSSYER
1
;
Mohammed AZIZ
1
;
Hassane MEKHFI
1
;
Mohammed BNOUHAM
1
;
Michel FREDERICH
2
;
Abderrahim ZIYYAT
3
Author Information
1. Laboratory of Physiology, Genetics and Ethnopharmacology, Department of Biology, Faculty of Sciences, Mohammed I University, Oujda 60000, Morocco.
2. Laboratory of Pharmacognosy, Center for Interdisciplinary Research on the Medicines (CIRM), University of Liège, Liège B-4000, Belgium.
3. Laboratory of Physiology, Genetics and Ethnopharmacology, Department of Biology, Faculty of Sciences, Mohammed I University, Oujda 60000, Morocco. Electronic address: ziyyat@yahoo.fr.
- Publication Type:Journal Article
- Keywords:
Ca(2+)-activated potassium channels;
Chenopodiaceae;
Chenopodium ambrosioides;
Complementary and alternative medicine;
High-performance liquid chromatography;
Plant;
Thin-layer chromatography;
Vasorelaxation
- From:
Journal of Integrative Medicine
2019;17(2):115-124
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:This study aims to evaluate the vasodilatory effect of Chenopodium ambrosioides on the isolated rat aorta, and to explore its mechanism of action.
METHODS:The vasorelaxant effect and the mode of action of various extracts from the leaves of C. ambrosioides were evaluated on thoracic aortic rings isolated from Wistar rats. In addition, ethyl acetate and methanol fractions were analyzed, using thin-layer chromatography and high-performance liquid chromatography techniques, for their polyphenolic content.
RESULTS:The various active extracts of C. ambrosioides at four concentrations (10, 10, 10 and 1 mg/mL) relaxed the contraction elicited by phenylephrine, in a concentration-dependent manner. This effect seems to be endothelium-dependent, since the vasodilatory effect was entirely absent in denuded aortic rings. The vasorelaxant effect of the methanol fraction (MF) of C. ambrosioides at 1 mg/mL was also inhibited by atropine and tetraethylammonium. This effect remained unchanged by Nω-nitro-l-arginine methyl ester hydrochloride and glibenclamide. The preliminary phytochemical analysis showed that the leaves of C. ambrosioides are rich in phenolic and flavonoid derivatives.
CONCLUSION:These results suggest that the MF of C. ambrosioides produces an endothelium-dependent relaxation of the isolated rat aorta, which is thought to be mediated mainly through stimulation of the muscarinic receptors, and probably involving the opening of Ca-activated potassium channels.
