Antioxidant activity of dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats.
10.1016/j.joim.2019.09.005
- Author:
Gaëtan Olivier FANKEM
1
;
Michel Archange FOKAM TAGNE
2
;
Paul Aimé NOUBISSI
3
;
Angèle FOYET FONDJO
4
;
Idrice KAMTCHOUING
1
;
Adela NGWEWONDO
5
;
Henri WAMBE
6
;
Joseph NGAKOU MUKAM
1
;
René KAMGANG
7
,
8
Author Information
1. Animal Physiology Laboratory, Faculty of Science, University of Yaoundé I, 237, Cameroon.
2. Department of Biological Science, Faculty of Science, University of Ngaoundéré, 237, Cameroon. Electronic address: fm_archange@yahoo.fr.
3. Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, 237, Cameroon.
4. Department of Applied Sciences for Health, Higher Institute of Applied Sciences, University Institute of Gulf of Guinea, 237, Cameroon.
5. Laboratory of Endocrinology and Radioisotopes, Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé 237, Cameroon.
6. Department of Biological Science, Faculty of Science, University of Dschang, 237, Cameroon.
7. Animal Physiology Laboratory, Faculty of Science, University of Yaoundé I, 237, Cameroon
8. Laboratory of Endocrinology and Radioisotopes, Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé 237, Cameroon.
- Publication Type:Journal Article
- Keywords:
Antioxidant stress;
Dichrocephala integrifolia;
Hematology;
Salmonella typhi;
Typhoid fever
- From:
Journal of Integrative Medicine
2019;17(6):438-445
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Infectious diseases such as typhoid fever lead to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work evaluated the antioxidant activity and histopathological effects of the dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats.
METHODS:The S. typhi-infected rats concurrently received daily doses of D. integrifolia extract at doses of 25, 50 and 100 mg/kg body weight or ciprofloxacin (5 mg/kg body weight) for 15 days. Body temperature was measured daily during infection and treatment periods. At the end of treatment period, the animals were sacrificed and biological responses including hematological parameters, superoxide dismutase and catalase activities, and glutathione, malondialdehyde and nitric oxide concentrations were evaluated.
RESULTS:The elevated body temperature induced by infection was significantly decreased in animals treated with 25, 50 or 100 mg/kg of the extract. Platelet levels decreased slightly in infected rats, while treatment with the dichloromethane fraction of D. integrifolia significantly increased platelet levels; this response was greater than that elicited by ciprofloxacin. The doses of 50 and 100 mg/kg of the dichloromethane fraction of D. integrifolia notably decreased monocyte and neutrophil values. Activity of superoxide dismutase and catalase and levels of glutathione in the tissues of treated animals were increased significantly (P < 0.01), while malondialdehyde and nitric oxide levels were significantly decreased (P < 0.01), following treatment with the dichloromethane fraction of D. integrifolia.
CONCLUSION:The results of this study show that the dichloromethane fraction of D. integrifolia has protective effects against a series of pathological conditions initiated by oxidation and tissue damage in the course of a S. typhi infection.
