A design of raster plot for illustrating dynamic neuronal activity during deep brain stimulation.
10.7507/1001-5515.201805016
- Author:
Lu HUANG
1
;
Zhaoxiang WANG
1
;
Zhouyan FENG
2
Author Information
1. Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, P.R.China.
2. Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, P.R.China.fengzhouyan@139.com.
- Publication Type:Journal Article
- Keywords:
dynamic response;
high frequency stimulation;
latency;
phase-locking;
raster plots;
spike
- MeSH:
Action Potentials;
Animals;
Axons;
physiology;
CA1 Region, Hippocampal;
physiology;
Deep Brain Stimulation;
Neurons;
physiology;
Rats
- From:
Journal of Biomedical Engineering
2019;36(2):177-182
- CountryChina
- Language:Chinese
-
Abstract:
Deep brain stimulation (DBS), which usually utilizes high frequency stimulation (HFS) of electrical pulses, is effective for treating many brain disorders in clinic. Studying the dynamic response of downstream neurons to HFS and its time relationship with stimulus pulses can reveal important mechanisms of DBS and advance the development of new stimulation modes (e.g., closed-loop DBS). To exhibit the dynamic neuronal firing and its relationship with stimuli, we designed a two-dimensional raster plot to visualize neuronal activity during HFS (especially in the initial stage of HFS). Additionally, the influence of plot resolution on the visualization effect was investigated. The method was then validated by investigating the neuronal responses to the axonal HFS in the hippocampal CA1 region of rats. Results show that the new design of raster plot is able to illustrate the dynamics of indexes (such as phase-locked relationship and latency) of single unit activity (i.e., spikes) during periodic pulse stimulations. Furthermore, the plots can intuitively show changes of neuronal firing from the baseline before stimulation to the onset dynamics during stimulation, as well as other information including the silent period of spikes immediately following the end of HFS. In addition, by adjusting resolution, the raster plot can be adapted to a large range of firing rates for clear illustration of neuronal activity. The new raster plot can illustrate more information with a clearer image than a regular raster plot, and thereby provides a useful tool for studying neuronal behaviors during high-frequency stimulations in brain.
