Comprehensive analysis of the aberrantly expressed profiles of lncRNAs, miRNAs and the regulation network of the associated ceRNAs in clear cell renal cell carcinoma.
10.7507/1001-5515.201801057
- Author:
Wanting HOU
1
,
2
;
Qiulin TANG
2
;
Feng BI
1
,
3
Author Information
1. Department of Abdominal Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China
2. Laboratory of Molecular Targeted Therapy in Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
3. Laboratory of Molecular Targeted Therapy in Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.bifeng@scu.edu.cn.
- Publication Type:Journal Article
- Keywords:
The Cancer Genome Atlas;
clear cell renal cell carcinoma;
competing endogenous RNA;
long noncoding RNA;
microRNA
- MeSH:
Carcinoma, Renal Cell;
genetics;
Humans;
Kidney Neoplasms;
genetics;
MicroRNAs;
genetics;
RNA, Long Noncoding;
genetics;
Transcriptome
- From:
Journal of Biomedical Engineering
2019;36(2):267-273
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the differential expression profiles of the lncRNAs, miRNAs, mRNAs and ceRNAs, and their implication in the prognosis in clear cell renal cell carcinoma (CCRCC), the large sample genomics analysis technologies were used in this study. The RNA and miRNA sequencing data of CCRCC were obtained from The Cancer Genome Atlas (TCGA) database, and R software was used for gene expression analysis and survival analysis. Cytoscape software was used to construct the ceRNA network. The results showed that a total of 1 570 lncRNAs, 54 miRNAs, and 17 mRNAs were differentially expressed in CCRCC, and most of their expression levels were up-regulated (false discovery rate < 0.01 and absolute log fold change > 2). The ceRNA regulatory network showed the interaction between 89 differentially expressed lncRNAs and 9 differentially expressed miRNAs. Further survival analysis revealed that 38 lncRNAs (including COL18A1-AS1, TCL6, LINC00475, UCA1, WT1-AS, HOTTIP, PVT1, etc.) and 2 miRNAs (including miR-21 and miR-155) were correlated with the overall survival time of CCRCC ( < 0.05). Together, this study provided us several new evidences for the targeted therapy and prognosis assessment of CCRCC.
