Construction of real-time polymerase chain reaction detection for infection-related cytokines of tree shrew.
10.7507/1001-5515.201801036
- Author:
Xiao LI
1
;
Wenkuan LIU
2
;
Shuyan QIU
1
;
Duo XU
1
;
Zhichao ZHOU
1
;
Xingui TIAN
1
;
Chi LI
1
;
Shujun GU
1
;
Rong ZHOU
1
Author Information
1. State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, P.R.China.
2. State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, P.R.China.ahlwk2000-2004@163.com.
- Publication Type:Journal Article
- Keywords:
cytokine;
real-time polymerase chain reaction;
tree shrew
- MeSH:
Animals;
Cytokines;
analysis;
Real-Time Polymerase Chain Reaction;
Shrews
- From:
Journal of Biomedical Engineering
2019;36(3):407-413
- CountryChina
- Language:Chinese
-
Abstract:
Tree shrew is a novel and high-quality experimental animal model. In this study, the real-time polymerase chain reaction methods were established to detect infection-related cytokines interleukin-6 (IL-6), IL-8, IL-10, IL-17A, interferon-γ (IFN-γ) and housekeeping gene glyceraldehyde-phosphate dehydrogenase ( ) of tree shrew. The results indicated that the establised methods had good specificity. The high point of the linear range of these reagents reached 1 × 10 copies, and the low points ranged from 10 copies (IL-6, IL-17A), 100 copies (IL-10, ) to 1 000 copies (IL-8, IFN-γ). In this interval, the linear correlation coefficient of each reagent was greater than 0.99. The lowest detectable values of IL-6, IL-8, IL-10, IL-17A, IFN-γ and were 8, 8, 4, 8, 128 and 4 copies, respectively. The results showed that the established detection methods had good specificity, sensitivity and wide linear range. The methods were suitable for detection of multiple concentration range samples, and could be used for the subsequent studies of tree shrew cytokines.
