Research of simulated microgravity regulate MC3T3-E1 cells differentiation through the nuclear factor-kappa B signaling pathway.
10.7507/1001-5515.201801019
- Author:
Biao HAN
1
;
Yang ZHANG
1
;
Hao LI
1
;
Shuping WEI
1
;
Ruixin LI
1
;
Xizheng ZHANG
2
Author Information
1. Institute of Medical Service and Technology, Academy of System Engineering, Academy of Military Sciences, Tianjin 300161, P.R.China.
2. Institute of Medical Service and Technology, Academy of System Engineering, Academy of Military Sciences, Tianjin 300161, P.R.China.z56787@sohu.com.
- Publication Type:Journal Article
- Keywords:
bone morphogenetic proteins;
nuclear factor-kappa B signaling pathway;
osteoblast;
osteoblast differentiation;
simulated microgravity
- MeSH:
3T3 Cells;
Animals;
Cell Differentiation;
Mice;
NF-kappa B;
physiology;
Osteoblasts;
Signal Transduction;
Weightlessness Simulation
- From:
Journal of Biomedical Engineering
2019;36(3):421-427
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we aim to investigat the effect of microgravity on osteoblast differentiation in osteoblast-like cells (MC3T3-E1). In addition, we explored the response mechanism of nuclear factor-kappa B (NF-κB) signaling pathway to "zero- " in MC3T3-E1 cells under the simulated microgravity conditions. MC3T3-E1 were cultured in conventional (CON) and simulated microgravity (SMG), respectively. Then, the expression of the related osteoblastic genes and the specific molecules in NF-κB signaling pathway were measured. The results showed that the mRNA and protein levels of alkaline phosphatase (ALP), osteocalcin (OCN) and type Ⅰ collagen (CoL-Ⅰ) were dramatically decreased under the simulated microgravity. Meanwhile, the NF-κB inhibitor α (IκB-α) protein level was decreased and the expressions of phosphorylation of IκB-α (p-IκB-α), p65 and phosphorylation of p65 (p-p65) were significantly up-regulated in SMG group. In addition, the IL-6 content in SMG group was increased compared to CON. These results indicated that simulated microgravity could activate the NF-κB pathway to regulate MC3T3-E1 cells differentiation.
