Effects of respiratory syncytial virus infection on epidermal growth factor receptor, tight junction association proteins and mucin in airway epithelial cells.
- Author:
Juan-Juan LIU
1
;
Ting ZHANG
;
Yu-Mei MI
Author Information
1. Department of Infectious Diseases, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China. 6512035@zju.edu.cn.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Epithelial Cells;
ErbB Receptors;
Humans;
Mucin 5AC;
Respiratory Syncytial Virus Infections;
Tight Junction Proteins;
Tight Junctions
- From:
Chinese Journal of Contemporary Pediatrics
2019;21(3):294-299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of respiratory syncytial virus (RSV) infection on epidermal growth factor receptor (EGFR), tight junction association proteins and mucin in the human airway epithelial cells.
METHODS:Human airway epithelial cells NCI-H292 were randomly treated by ultraviolet light-inactivated RSV (control group) or thawed RSV (RSV infection group). After 48 hours of treatment, the protein levels of occludin, E-cadherin, phosphorylated EGFR and EGFR in NCI-H292 cells were measured by Western blot. The distribution and expression levels of occludin and E-cadherin in NCI-H292 cells were examined by immunofluorescence technique. The expression levels of MUC5AC mRNA in NCI-H292 cells were assessed by RT-PCR.
RESULTS:The protein levels of occludin and E-cadherin were significantly reduced in the RSV infection group compared with the control group (P<0.05). The protein levels of phosphorylated EGFR and EGFR increased significantly in the RSV infection group compared with the control group (P<0.05). The MUC5AC mRNA levels also increased significantly in the RSV infection group compared with the control group (P<0.05).
CONCLUSIONS:RSV may down-regulate the tight junction association proteins and up-regulate the expression of MUC5AC in airway epithelial cells, which contributes to epithelial barrier dysfunction. EGFR phosphorylation may play an important role in regulation of airway barrier.
