Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits.
10.12047/j.cjap.5484.2018.001
- Author:
Yang-Yang LI
1
;
Lu SHI
2
;
Yan-Meng ZHANG
2
;
Chan-Juan XIAO
1
;
Hong-Tao LIU
1
Author Information
1. Shanghai Jiao Tong University Underwater Engineering Insititute Co. Ltd, Shanghai 200231, China.
2. Shanghai Jiao Tong University, Institute of Underwater Technology, Shanghai 200231, China.
- Publication Type:Journal Article
- Keywords:
Trimix conventional diving;
inflammatory reaction;
oxidative stress;
wet/dry ratio
- MeSH:
Animals;
Catalase;
Diving;
physiology;
Glutathione;
Helium;
Inflammation;
Interleukin-6;
Interleukin-8;
Malondialdehyde;
Matrix Metalloproteinase 9;
Nitrogen;
Oxidative Stress;
Oxygen;
Peroxidase;
Rabbits;
Superoxide Dismutase;
Tumor Necrosis Factor-alpha
- From:
Chinese Journal of Applied Physiology
2018;34(1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of simulated 100 m Trimix conventional diving on tissue inflammatory cytokines in rabbits.
METHODS:Eight New Zealand rabbits were performed a simulated 100 m Trimix conventional diving program which was established according to the Haldane theory. The expression levels of interferon-gamma(IFN-), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), myeloperoxidase(MPO) and matrix metallo proteinase-9 (MMP-9) in rabbits lung and brain tissues were detected by Elisa after diving decompression. The tissue wet/dry ratio was calculated. The serum levels of superoxide dismutase (SOD),glutathione(GSH), catalase(CAT), malondiadehyde(MDA) and lipid peroxide(LPO) were detected by Elisa method in rabbits before and after diving.
RESULTS:The expressions of IFN-, TNF-α, IL-6, IL-8, MPO and MMP-9 in simulated diving group rabbits were significantly increased compared with the intact group(<0.05, <0.01); the simulated diving rabbits tissues wet/dry ratio had no significant changes compared with the intact group. After diving, the activities of SOD and GSH were decreased significantly (<0.01), while the contents of CAT, MDA and LPO were increased significantly (<0.01).
CONCLUSIONS:The simulated 100 m Trimix conventional diving had significant impact on oxidative stress and inflammatory reaction in rabbits, the results of wet/dry ratio showed that the diving rabbits had no tissue edema after decompression.
