Effects of CHL1 deficiency,a cell adhesion molecule,on the inflammatory bowel disease.
10.12047/j.cjap.5567.2018.002
- Author:
Xiao-Meng WANG
1
;
Tong ZHAO
1
;
Xiang CHENG
1
;
Ning GUO
1
;
Ling-Ling ZHU
1
;
Ming SHI
2
;
Kui-Wu WU
1
Author Information
1. Department of Brain Science and Stress, Institute of Cognition and Brain Sciences, Beijing 100850.
2. Jiangsu Province Cancer Biotherapy Institute, Xuzhou 221004, China.
- Publication Type:Journal Article
- Keywords:
CHL1;
DSS;
IBD
- MeSH:
Animals;
Cell Adhesion Molecules;
deficiency;
genetics;
Colitis;
chemically induced;
genetics;
Colon;
pathology;
Dextran Sulfate;
Disease Models, Animal;
Mice;
Mice, Inbred C57BL;
Random Allocation
- From:
Chinese Journal of Applied Physiology
2018;34(1):4-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of deficiency of CHL1 in inflammatory bowel disease (IBD).
METHODS:Dextran Sulfate Sodium (DSS)-induced colitis model was used to study the effects of deficiency of CHL1 on the development of IBD. Ten CHL1(+/+) mice in C57/BL6 background were randomly divided into CHL1(+/+) group and DSS-induced CHL1(+/+) group. Ten CHL1(-/-) mice in C57/BL6 background were randomly divided into CHL1(-/-) group and DSS-induced CHL1(-/-) group. DSS-induced CHL1(+/+) group and DSS-induced CHL1(-/-)group were fed with 1.5% DSS for 7 days, and then drinking distilled water for 2 days. CHL1(+/+) group and CHL1(-/-) group as control group were fed with distilled water for 9 days. The changes of weight, survival, fecal blood and the change of colon length in this study were observed.
RESULTS:On the 7 day, the weight of DSS-induced CHL1(-/-) group were reduced significantly, and DSS-induced CHL1(-/-) group had extreme mortality on the 9th day. The fecal blood of DSS-induced CHL1(-/-) group also had higher score than that of DSS-induced CHL1(+/+) group. In the DSS-induced CHL1(-/-) group,the length of colon was shortened obviously.
CONCLUSIONS:The loss of CHL1 aggravates the development of IBD.
