Effect of dexmedetomidine on apoptosis and CHOP in hypoxia/reoxygenation injury A549 cell.
10.12047.j.cjap.5554.2018.034
- Author:
Zi-Yin LUO
1
;
Hui GAO
1
;
Bing-Qian XIANG
1
;
Xiao-Xiao QIU
1
;
Yong-Yue DAI
1
;
Wan-Tie WANG
1
Author Information
1. Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou 325035.
- Publication Type:Journal Article
- Keywords:
A549 cell;
CHOP;
apoptosis;
dexmedetomidine;
hypoxia/reoxygenation injury
- MeSH:
A549 Cells;
Apoptosis;
Cell Hypoxia;
Dexmedetomidine;
pharmacology;
Humans;
Transcription Factor CHOP;
physiology
- From:
Chinese Journal of Applied Physiology
2018;34(2):137-142
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the effects of dexmedetomidine (Dex) on injury of A549 cells induced by hypoxia/reoxygenation(H/R)and the influence of C/EBP homologous protein (CHOP) expression.
METHODS:Logarithmic growth phase A549 cells(it originated from alveolar type Ⅱ epithelial cell line) were randomly divided into 4 groups (=10):normoxic control group (N), Dex group (D), hypoxia/reoxygenation group (H), hypoxia/reoxygenation + Dex group(HD). At the beginning of modeling, 1 nmol/L Dex was puted into D and HD groups. N and D groups were cultured in the normoxic incubator for 30 h. H and HD group were incubated in the anoxic cultivation for 6 h, fo llowed by normoxic culture for 24 h. Then A549 cells were observed under the inverted microscope to observe the morphological changes. Cell activity was detected by cell counting Kit-8(CCK-8) and the apoptosis index(AI) was detected by in situ end labeling (TUNEL) method. The expression of CHOP、glucose-regulated protein of molecular weight 78 kDa (Grp78)、cysteinyl aspirate-specificprotease-3 (caspase-3) protein and CHOP、Grp78 mRNA were detected by Western blot and RT-PCR.
RESULTS:Compared with N group, the number of adherent cells in H group decreased significantly, and cell morphology changed. The absorbance value in H group decreased obviously (<0. 01). The AI value and expression of CHOP, Grp78, caspase-3 proteins and CHOP, Grp78 mRNA were significantly increased (<0.01). Compared with H group, the cell damage in HD group was decreased, the absorbance value increased (<0.01), the number of apoptosis cells decreased relatively (<0.01), the expression of CHOP, caspase-3 protein and CHOP mRNA decreased (<0. 01).
CONCLUSIONS:Dex has notable effects against H/R injury, which may be related to effective inhibition of apoptosis mediated by the CHOP's signal path.
