Effect of Yuyin Ruangan Granule on TGF-β1 expression in hepatic fibrosis rats.
10.12047/j.cjap.5593.2018.041
- Author:
Zhi-Wang WANG
1
;
Xiao-Yan FU
1
;
Xiao-Li CHENG
1
;
Xiao-Ying BAO
2
;
Xue-Feng LIU
1
;
Hai-Jing DUAN
1
Author Information
1. Gansu University of Chinese Medicine, Lanzhou 730000.
2. Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
Yuyin Ruangan Granule (YRG);
carbon tetrachloride;
hepatic fibrosis;
rat;
β1-transformating growth factor (TGF-β1)
- MeSH:
Animals;
Carbon Tetrachloride;
Drugs, Chinese Herbal;
pharmacology;
Liver;
drug effects;
metabolism;
Liver Cirrhosis;
chemically induced;
drug therapy;
metabolism;
Random Allocation;
Rats;
Rats, Wistar;
Transforming Growth Factor beta1;
metabolism
- From:
Chinese Journal of Applied Physiology
2018;34(2):169-172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the preventive and therapeutic action of Yuyin Ruangan Granule (YRG, Traditional Chinese Medicine) in hepatic fibrosis rats model and its effect on transforming growth factor-β1 (TGF-β1) expression.
METHODS:The Wistar rats were randomly divided into 6 group (=10), and the model of hepatic fibrosis rats was established by subcutaneous injected with carbon tetrachloride (CCL4), fed on high-fat diet and 20% ethanol for 6 weeks, to survey the effect and mechanism of YRG preventing hypatic fibrosis by detecting liver function (the activity of alanine aminotransferase(ALT), aspartate aminotransferase(AST), etc.) of liver fibrosis rats, liver fibrosis indicators (hyaluronic acid, Ⅲ procollagen, type IV collagen, laminin and hepatic pathology, etc.), and TGF-β1 expression in liver tissue after 6 weeks treated with YRG through intragastric administration (q. d.).
RESULTS:At the 7 week, fibrotic lesions appears distinctly in liver tissue of model group compared with control group (<0.01), YRG of 6.2~28.8 g/kg could significantly decrease hepatic index, ALT and AST activities, content of hyaluronic acid(HA), Ⅲ procollagen (PCⅢ), type Ⅳ collagen(C-Ⅳ), laminin (LN) in serum, relieve liver fibrosis pathological changes and inhibit TGF-β1 expression in fibrotic liver tissue (<0.05, <0.01).
CONCLUSIONS:YRG has significantly preventive effects on liver fibrosis rats model, and it may be one of its mechanisms to inhibit expression of TGF-β1.
