The therapeutic effects of Yougui pill on knee osteoarthritis and the expression of Wnt signal pathway related factors in rats.
10.12047/j.cjap.5603.2018.043
- Author:
Chun-Lu YAN
1
;
Sheng-Hua LI
2
;
Fang-Yu AN
1
;
Yong-Qi LIU
3
;
Peng-Fei XIA
4
;
Zheng-Min MA
2
;
Yan-Qiang NIU
5
;
Peng-Yao LIU
2
Author Information
1. Teaching Experiment Training Center, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China.
2. Clinical College of traditional Chinese Medicine, Lanzhou 730000, China.
3. Department of Microbiology and Immunology, Lanzhou 730000, China.
4. Scientific Research Experiment Center, Lanzhou 730000, China.
5. Clinical College, Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
Wnt signal pathway;
Yougui pill;
osteoarthritis of knee;
protection funtion;
rats
- MeSH:
Animals;
CCN Intercellular Signaling Proteins;
metabolism;
Drugs, Chinese Herbal;
pharmacology;
Glucosamine;
pharmacology;
Intercellular Signaling Peptides and Proteins;
metabolism;
Osteoarthritis, Knee;
drug therapy;
Proto-Oncogene Proteins;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Wnt Signaling Pathway;
Wnt1 Protein;
metabolism;
beta Catenin;
metabolism
- From:
Chinese Journal of Applied Physiology
2018;34(2):177-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the effects of Yougui pill (Traditional Chinese Medicine) on the related factors of Wnt signal pathway of rats with knee osteoarthritis (KOA), and explore its protective mechanism.
METHODS:Sixty SPF SD rats were randomly divided into the sham-operative group, model group, glucosamine sulfate group, high-dose, middle-dose, low-dose of Yougui pill treated group (=10). KOA model was established by modified Hulth method for six weeks. The rats in the high, middle and low-dose of Yougui pill group were treated with Yougui pills at the doses of 20,10 and 5 g/kg respectively by gastrogavage once a day for 8 weeks, while equal volume of normal saline was given to those in the sham and model control group and an equal volume of glucosamine sulfate (1.7 g/kg·d) was given to those in glucosamine sulfate group for 8 weeks. The knee joint was removed after the last dose of drug. The pathological changes of cartilaginous tissues were observed under a microscope. The mRNA levels of Dickkopf homolog 1(DKK1), Wnt induced secreted protein 1(WISP1), Wnt1, low density lipoprotein receptor related protein 5(LRP5) and beta -catenin in rats cartilaginous tissues were analyzed by using RT-PCR method, and the protein contents of DKK1, WISP1, Wnt1, LRP5 and beta-catenin in cartilaginous tissues were detected by Western blot.
RESULTS:Compared with the sham group, the articular cartilage was severely damaged, the Mankin score was increased significantly (<0. 05), the mRNA and protein expression levels of DKK1 in cartilaginous tissue were markedly decreased(<0.05), while those of WISP, Wnt1, LRP5 and beta-catenin were increased significantly in model group(<0.05). Compared with model group, the articular cartilage lesions was light (<0.05), the Mankin Score was decreased significantly(<0.05), and the mRNA and protein levels of DKK1 in cartilaginous tissue were increased(<0.05), while those of WISP, Wnt1, LRP5 and beta-catenin were decreased in Yougui pill high-dose group and glucosamine sulfate group (<0.05).
CONCLUSIONS:Yougui pill has protective effects on the KOA by inhibiting the expressions of WISP, Wnt1, LRP5, beta-catenin and increasing the expression of DKK1 cytokine in the Wnt signaling pathway.
