High sensitive ELISA for detection of atrazion.
10.12047/j.cjap.5582.2018.045
- Author:
Xiao-Li LI
1
;
Ting-Ting WANG
1
;
Kai LIU
1
;
Bao-An NING
1
;
Xin-Hua MA
1
;
Nan LIU
1
;
Guo-Rong OU
1
;
Zhi-Xian GAO
1
;
Zhong-Wen LIU
1
Author Information
1. Institute of Environmental and Operational Medicine, Military Medical Research Institute of Military Academy Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin 300050, China.
- Publication Type:Journal Article
- Keywords:
ELISA;
atrazine;
detection
- MeSH:
Animals;
Atrazine;
analysis;
Chromatography, High Pressure Liquid;
Enzyme-Linked Immunosorbent Assay;
Sensitivity and Specificity
- From:
Chinese Journal of Applied Physiology
2018;34(2):187-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To set up ELISA for detection of atrazine with high precision.
METHODS:The reaction condition of indirect-ELISA was optimized, including atrazine-ovalbumin(AT-OVA) concentration and primary antibody concentration, organic solvent, goat anti-rat immunoglobin G-horseradish peroxidase(IgG-HRP) concentration. The actual samples were detected by the ELISA method established in our laboratory. Then the ELISA method was compared with the HPLC.
RESULTS:The specification curve of indirect-ELISA was set up after optimization. The relation coefficient R=0.9958. The limit of detection (LOD) was 1.972 ng/ml. The percent recovery of the actual samples was in range of 80%~120%. The ELISA detection sensitivity was higher than the HPLC in the range of 0 ng/ml~6 ng/ml atrazine.
CONCLUSIONS:The ELISA to detect atrazine has good specificity and high precision. And it can be applied in testing real atrazine samples replacing of the large-scale instrument.
