Placenta-derived mesenchymal stem cells with HLA-G positive expression induce Treg .
10.12047/j.cjap.5621.2018.090
- Author:
Gui-Yu CUI
1
;
Jian BAI
2
;
Lan-Ying MIAO
2
;
Da-Yong LIN
2
;
Hong LIU
2
;
Ya-Li LI
3
;
Xi-Cheng LIU
3
Author Information
1. Medical School of Inner Mongolia University for Nationalities, Tongliao 028000.
2. Liao Ning University of Traditional Chinese Medicine, Shenyang 110847.
3. Department of Anesthesiology, Shenzhen People's Hospital, Shenzhen 518020.
- Publication Type:Journal Article
- Keywords:
T lymphocyte;
human leucocyte antigen-G;
placenta-derived mesenchymal stem cell;
regulatory T lymphocyte
- MeSH:
Female;
Forkhead Transcription Factors;
HLA-G Antigens;
Humans;
Mesenchymal Stem Cells;
Placenta;
Pregnancy;
T-Lymphocytes, Regulatory
- From:
Chinese Journal of Applied Physiology
2018;34(5):396-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study placenta-derived mesenchymal stem cells with HLA-G (Human Leukocyte Antigen, HLA-G) positive expression induce Treg (regulatory T cell, Treg) in vitro.
METHODS:placenta-derived mesenchymal stem cells were separated from neonatal placenta; PEGFP - N1 -HLA-G plasmid was transfected in placenta-derived mesenchymal stem cells by liposome transfection.The cells were divided into 3 groups including control group, PEGFP-N1 group and PEGFP-N1-HLA-G group, 5 complex walls in each group. Expression of HLA-G protein was detected by Western Blotting; after identification of cells, healthy human peripheral blood CD4 T lymphocytes were cultured with placenta-derived mesenchymal stem cells with HLA-G positive expression, and the ratio of CD4CD25Foxp3Treg in T lymphocytes was accounted.
RESULTS:After transfection of PEGFP-N1-HLA-G, the placenta-derived mesenchymal stem cells can express HLA-G protein significantly, compared with the control group and PEGFP - N1 group (<0.01). After HLA-G positive placenta-derived mesenchymal stem cells and CD4 + T lymphocytes were cultured for 24 h, the ratio of CD4CD25Foxp3Treg in T lymphocytes was (16.41±0.94)%. After HLA - G positive placenta-derived mesenchymal stem cells and CD4 T lymphocytes were cultured for 48 h, the ratio of CD4CD25Foxp3Treg in T lymphocytes was (16.46±0.59)% significantly, compared with the control group and PEGFP - N1 group (<0.01).
CONCLUSIONS:Placenta-derived mesenchymal stem cells modified by HLA-G gene can effectively induce CD4CD25Foxp3Treg in vitro.
