Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress.
10.12047/j.cjap.5624.2018.092
- Author:
Xin-Xin CHEN
1
;
Xiao-Yun ZHANG
2
;
Yu-Zhen DING
1
;
Xin LI
2
;
Xiu-Mei GUAN
2
;
Hong LI
2
;
Min CHENG
2
;
Xiao-Dong CUI
2
Author Information
1. College of Biological Science and Technology, Weifang Medical University, Weifang 261053, China.
2. Clinical Medical College, Weifang Medical University, Weifang 261053, China.
- Publication Type:Journal Article
- Keywords:
co-culture;
ecological niche;
endothelial progenitor cells;
hepatic stellate cells;
shear stress
- MeSH:
Actins;
Apoptosis;
Cell Proliferation;
Cells, Cultured;
Collagen Type I;
Endothelial Progenitor Cells;
Hepatic Stellate Cells
- From:
Chinese Journal of Applied Physiology
2018;34(5):404-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of endothelial progenitor cells (EPCs) under shear stress on the biological function such as proliferation, adhesion, migration, apoptosis and expression of α-smooth muscle actin (α-SMA), collagen-I and collagen-Ⅲ of hepatic stellate cells (HSCs).
METHODS:HSCs and EPCs were inoculated into the upper and lower layers of the co-culture chamber respectively and co-incubated for 24 hours. Then, 12 dyne/cm shear stress was applied to EPCs cells for another 24 hours. After that, proliferation, adhesion, migration and apoptosis of HSCs were detected by cell counting kit-8 (CCK-8) kit, cell adherent assay, Boyden cell migration assay and flow cytometry respectively. Fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expression of alpha -SMA, collagen I and collagen-Ⅲ in HSCs.
RESULTS:Under shear stress, EPCs ecological niche could obviously inhibit the proliferation, adhesion and migration of HSCs, promote the apoptosis of HSCs, and down-regulate the mRNA and protein expression of collagen-I, collagen-Ⅲ in HSC cells.
CONCLUSIONS:Under shear stress, EPCs ecological niche could inhibit the fibrosis development of HSCs to a certain extent.
