Effects of astragaloside-IV on the expression of inflammatory factor and proliferation of glomerular mesangial cells induced by angiotensin Ⅱ.
10.12047/j.cjap.5660.2018.094
- Author:
Li XIONG
1
;
Meng-Juan LYU
2
;
De-Yu DOU
2
;
Yu-Hong MA
1
Author Information
1. Department of Diagnostic, Wannan Medical College, Wuhu 241001, China.
2. Department of Central Lab, Wannan Medical College, Wuhu 241001, China.
- Publication Type:Journal Article
- Keywords:
astragaloside-IV;
monocyte chemoattractant protein-1;
rat glomerular mesangial cells;
reactive oxygen species
- MeSH:
Angiotensin II;
Blotting, Western;
Cell Proliferation;
Cells, Cultured;
Diabetic Nephropathies;
Humans;
Mesangial Cells;
Transforming Growth Factor beta1
- From:
Chinese Journal of Applied Physiology
2018;34(5):414-417 421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of astragaloside-IV (As-IV) on the expression of inflammatory factor and proliferation of glomerular mesangial cells (GMCs) induced by angiotensin Ⅱ(AngⅡ).
METHODS:The model of diabetic nephropathy(DN) was mimic by angiotensin Ⅱ (10mol/L)inducing GMCs injury. Then the GMCs were treated with As-IV at different concentrations(25,50,100 μmol/L)for 48 hours. The proliferation of GMCs was detected by MTT. The level of reactive oxidative species (ROS) was measured by flow cytometry. The expression of monocyte chemoattractant protein-1(MCP-1) protein in supernatant was detected by enzyme- linked immunosorbent assay (ELISA). The expression of transforming growth factor-β1(TGF-β1) in GMCs was measured by Western blot.
RESULTS:Compared with model group, the proliferation of GMCs was inhibited in As-IV group. As-IV decreased the level of intercellular ROS, down-regulated the secretion of MCP-1 and the expression of TGF-β1 proteins.
CONCLUSIONS:As-IV could inhibit cell proliferation and inflammatory factors expression on GMCs induced by AngⅡ.
