Effects of laser stimulation with different power on oxygen consumption rate of CC myoblasts in mice.
10.12047/j.cjap.5667.2018.095
- Author:
Yu FENG
1
;
Ze-Peng CHENG
1
;
Peng SUN
1
;
Reng-Fei SHI
1
Author Information
1. Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China.
- Publication Type:Journal Article
- Keywords:
C2C12 cells;
PGC-1α;
laser irradiation;
oxygen consumption rate
- MeSH:
Animals;
Cell Line;
Mice;
Myoblasts;
Oxidation-Reduction;
Oxygen Consumption;
Transcription Factors
- From:
Chinese Journal of Applied Physiology
2018;34(5):418-421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in CC myoblasts following different doses.
METHODS:CC cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm. Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot.
RESULTS:Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(<0.05). MyoD and PGC-1α protein expressions were also increased(<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(<0.05).
CONCLUSIONS:650 nm laser irradiation that dose is 0.4 J/cm enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.
