Investigation on intestinal absorption ingredients and their absorption characteristics in Pterocephali Herba by everted intestinal sac method.
10.19540/j.cnki.cjcmm.20190628.204
- Author:
Wen-Xiang WANG
1
;
Jin-Song SU
1
;
Meng YU
2
;
Xiu-Hua LIU
1
;
Hong-Jun YANG
3
;
Zhong-Mei ZOU
2
;
Yi ZHANG
1
;
Ce TANG
1
Author Information
1. Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
2. Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
3. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Pterocephali Herba;
Tibetan medicine;
UPLC-PDA;
everted intestinal sac method;
intestinal absorption
- MeSH:
Animals;
Caprifoliaceae;
chemistry;
Drugs, Chinese Herbal;
pharmacokinetics;
Intestinal Absorption;
Intestines;
Plant Extracts;
pharmacokinetics;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2019;44(15):3170-3177
- CountryChina
- Language:Chinese
-
Abstract:
The intestinal absorption characteristics of ten iridoid glycosides and phenolic acids in the Pterocephali Herba were evaluated via rat intestinal valgus model. The intestinal sac fluids at different time after administration of high,medium and low concentrations of Pterocephali Herba extract were collected and ten chemical components in fluid samples were detected by UPLC-PDA. Accumulative absorbed doses( Q) and absorption rate constants( Ka) of ten chemical constituents were calculated,while proportions between Pterocephali Herba extract and intestinal absorption liquid were compared. The results showed that the intestinal absorption of 10 chemical components was linear absorption( R2>0. 9) at different concentrations,which accorded with the zero-order absorption rate. The absorption rate constant was related to the concentration of the drug and the intestinal site,which indicated that intestinal adsorption mechanism of the components were passive diffusion and active transport. Proportions of chemical constituents in intestinal sac fluid were different from those in Pterocephali Herba extract. Therefore,those ten chemical components in Pterocephali Herba extract can be absorbed in whole intestine. Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Pterocephali Herba extract effectively.
