Transcriptome analysis reveals candidate genes involved in flavonoid biosynthesis in Ziziphora bungeana.
10.19540/j.cnki.cjcmm.20190628.202
- Author:
Jiang HE
1
;
Yi-Mian MA
2
;
Wei-Jun YANG
3
;
Bo CHENG
3
;
Li-Nu-Er DI
3
;
Li-Na MA
4
;
Guan LI
4
Author Information
1. College of Life Science and Technology,Xinjiang University Urumqi 830046,China Key Laboratory of Uighur Medicine,Xinjiang Institute of Materia Medica Urumqi 830004,China.
2. Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
3. Key Laboratory of Uighur Medicine,Xinjiang Institute of Materia Medica Urumqi 830004,China.
4. College of Life Science and Technology,Xinjiang University Urumqi 830046,China.
- Publication Type:Journal Article
- Keywords:
Ziziphora bungeana;
biosynthesis;
flavonoids;
transcriptome;
unigene
- MeSH:
Flavonoids;
biosynthesis;
Gene Expression Profiling;
High-Throughput Nucleotide Sequencing;
Lamiaceae;
chemistry;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2019;44(15):3178-3186
- CountryChina
- Language:Chinese
-
Abstract:
Ziziphora bungeana is a kind of medicinal plants belongs to Labiatae,and it also a kind of geoherbs in Xinjiang. The main active ingredient linarin has a higher content in inflorescence than in other parts. In this study,high-throughput sequencing technology was used to reveal the transcriptome of the inflorescence of Z. bungeana,77 366 unigenes were acquired,of which 56 375 unigenes were annotated based on search of the database and classification. Through the analysis of metabolic pathways,sixty unigenes were probably encoding some enzymes involved in the flavonoid biosynthesis pathways. The contents of linarin in different parts were determined and the key genes were verified by qRT-PCR. The discovery provides the research basis for further analysis of the enzyme genes involved in the biosynthesis of the major flavonoid components in Z. bungeana.
