Isolation and identification of endophytic fungi from Huperzia serrata and their metabolites' inhibitory activities against acetylcholinesterase and anti-inflammatory activities.
10.19540/j.cnki.cjcmm.20190527.101
- Author:
Bo-Wen QI
1
;
Ting MO
2
;
Xin ZHANG
1
;
Ya-Ru YAN
1
;
Xi-Ping XU
1
;
Hong-Yun YANG
1
;
Xiao-Hui WANG
1
;
Jun LI
1
;
She-Po SHI
1
;
Xiao LIU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
2. Ningxia Hui Medicine Research Institute Yinchuan 750021,China.
- Publication Type:Journal Article
- Keywords:
Huperzia serrate;
ITS sequence;
acetylcholinesterase;
anti-inflammatory activities;
endophytic fungi
- MeSH:
Acetylcholinesterase;
Animals;
Anti-Inflammatory Agents;
isolation & purification;
pharmacology;
Ascomycota;
chemistry;
classification;
isolation & purification;
Cholinesterase Inhibitors;
isolation & purification;
metabolism;
Endophytes;
classification;
isolation & purification;
Huperzia;
microbiology;
Mice;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2019;44(15):3213-3220
- CountryChina
- Language:Chinese
-
Abstract:
A total of 27 endophytic fungal strains were isolated from Huperzia serrata,which were richly distributed in the stems and leaves while less distributed in roots. The 27 strains were identified by Internal Transcribed Spacer( ITS) r DNA molecular method and one of the strains belongs to Basidiomycota phylum,and other 26 stains belong to 26 species,9 general,6 families,5 orders,3 classes of Ascomycota Phylum. The dominant strains were Colletotrichum genus,belonging to Glomerellaceae family,Glomerellales order,Sordariomycetes class,Ascomycota Phylum,with the percentage of 48. 15%. The inhibitory activities of the crude extracts of 27 endophytic fungal strains against acetylcholinesterase( ACh E) and nitric oxide( NO) production were evaluated by Ellman's method and Griess method,respectively. Crude extracts of four fungi exhibited inhibitory activities against ACh E with an IC50 value of 42. 5-62. 4 mg·L~(-1),and some fungi's crude extracts were found to inhibit nitric oxide( NO) production in lipopolysaccharide( LPS)-activated RAW264. 7 macrophage cells with an IC50 value of 2. 2-51. 3 mg·L~(-1),which indicated that these fungi had potential anti-inflammatory activities.The chemical composition of the Et OAc extract of endophytic fungus HS21 was also analyzed by LCMS-IT-TOF. Seventeen compounds including six polyketides,four diphenyl ether derivatives and seven meroterpenoids were putatively identified.
