Study on quantification method of non-starch polysaccharides in Dendrobium catenatum.
10.19540/j.cnki.cjcmm.20190527.104
- Author:
Ya LI
1
;
Jing-Jing LIU
1
;
Xin-Feng ZHANG
1
;
Shi-Hua WU
2
;
Shao-Ping LI
3
;
Jin-Ping SI
1
Author Information
1. State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University Lin'an 311300,China.
2. Zhejiang University Hangzhou 310000,China.
3. University of Macau Macao 999078,China.
- Publication Type:Journal Article
- Keywords:
Dendrobium catenatum;
monosaccharide composition;
non-starch polysaccharides;
α-amylase treatment
- MeSH:
Dendrobium;
chemistry;
Monosaccharides;
analysis;
Phytochemicals;
analysis;
Polysaccharides;
analysis;
Starch;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(15):3221-3225
- CountryChina
- Language:Chinese
-
Abstract:
The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum. In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation. The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method. The contents of starches,non-starch polysaccharides and monosaccharides were analyzed. In addition,the system suitability was tested. Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20. 9%,58. 8% and 1. 6% respectively. The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable. Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum. Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.
