Comparative study on differences of Paeonia lactiflora from different habitats based on fingerprint and chemometrics.
10.19540/j.cnki.cjcmm.20190424.202
- Author:
Qiu-Long ZHAO
1
;
Xiao-Kun BIAN
1
;
Da-Wei QIAN
2
;
Ting ZHANG
1
;
Zhen-Hua ZHU
2
;
Sheng GUO
2
;
Hui YAN
1
;
Tuan-Jie WANG
3
;
Zhi-Peng CHEN
4
;
Jin-Ao DUAN
2
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine Nanjing 210023,China.
2. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China.
3. Kanion Pharmaceutical Co.,Ltd. Lianyungang 222001,China.
4. College of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China.
- Publication Type:Journal Article
- Keywords:
PLS-DA;
Paeonia lactiflora;
chemometrics;
difference component;
fingerprint
- MeSH:
China;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Ecosystem;
Paeonia;
chemistry;
Phytochemicals;
analysis;
Plant Roots;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(15):3316-3322
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to compare the differences of Paeonia lactiflora from different habitats by establishing fingerprint. The fingerprint of P. lactiflora was established by UPLC. The samples collected from Sichuan,Hebei,Henan,Shanxi and Anhui were analyzed. The common peaks were identified by UPLC-Q-TOF/MS. The relative peak area of the common peaks was analyzed through similarity evaluation system( 2012 edition) for chromatographic fingerprint of traditional Chinese medicine developed by the National Pharmacopoeia Commission. Twelve common peaks were obtained and ten components were identified by reference substance and literature comparison. The similarity of each sample to the reference fingerprint is greater than 0. 900. However,all samples were clearly divided into 5 groups according to habitats after PLS-DA analysis. The peaks 2,6( ethyl gallate),10( galloypaeoniflorin) and 12( benzoyl paeoniflorin) were found to be the main difference components between the samples from five different habitats through the VIP value map. The study found that the variety of ingredients in the different areas are basically similar. But there are some differences in the content of the four components. The results of this study can provide reference at choosing and utilizing P. lactiflora from different places comprehensively.
