Correlation between lipid-lowering efficacy and components of Pericarpium Citri Reticulatae.
10.19540/j.cnki.cjcmm.20190523.304
- Author:
Jing-Jing YU
1
;
Jie SU
1
;
Mei-Qiu YAN
1
;
Zhao-Huan LOU
1
;
Gui-Yuan LYU
1
Author Information
1. College of Pharmaceutical Science,Zhejiang Chinese Medical University Hangzhou 310053,China.
- Publication Type:Journal Article
- Keywords:
Pearson correlation analysis;
Pericarpium Citri Reticultae;
flavonones;
hyperlipidemia;
polymethoxyflavonoids(PMFs)
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Citrus;
chemistry;
Drugs, Chinese Herbal;
pharmacology;
Flavonoids;
pharmacology;
Hypolipidemic Agents;
pharmacology;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(15):3335-3342
- CountryChina
- Language:Chinese
-
Abstract:
Based on pharmacodynamics-component correlation analysis,the best effective part of hyperlipidemia of Pericarpium Citri Reticulatae( PCR) was screened out to confirm the possible constituents with the lipid regulating effect,in order to provide a basis for the development of new drugs. Hyperlipidemia rats induced by fat emulsion were used to screen out the best hypolipidemic parts of PCR with TC,LDL-c as the index. HPLC-ESI-MS were used to analyze common constituents of the different solvent extracts from PCR. The constituents were classified and identified based on the retention time,m/z and UV spectra. And the HPLC-DAD were used to determine the contents of flavonoids( narirutin,hesperidin,didymin,nobiletin,tangeretin,3,5,6,7,8,3',4'-heptemthoxyflavone).Correlation analysis was conducted on the constituents and efficacy with the method of SPSS ANOVA bivariate correlation. Five extracts could significantly decrease the content of TC,LDL-c of hyperlipemia rats induced by fat emulsion,and the best effective part were95% ethanol and ethyl acetate. There were 19 common peaks in five different solvent extracts from PCR,and 17 flavonoids were identified and classified,including 10 polymethoxyflavonoids and 7 other flavonoids. According to the raw material quantity,the order of content of flavonones arranged from high to low: n-butyl alcohol part> 95% ethanol part> water part> ethyl acetate part> petroleum ether part; and the order of PMFs arranged from high to low: n-butyl alcohol part≈95% ethanol part≈ethyl acetate part > petroleum ether part > water part. The decreased percentage of TC,LDL-c was positively correlated with 10 common PMFs constituents,which suggested that PMFs may be the effective components for reducing blood lipid.
