Effect of Tripterygium Glycosides Tablets on synovial angiogenesis in rats with type Ⅱ collagen induced arthritis.
10.19540/j.cnki.cjcmm.20190625.401
- Author:
Jing-Xia WANG
1
;
Chun-Fang LIU
1
;
Yi-Qun LI
1
;
Xiao-Hui SU
1
;
Li-Ling LIU
1
;
Ya-Ge TIAN
1
;
Jin-Xia WANG
1
;
Ke-Xin JIA
1
;
Na LIN
1
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medicine Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
HUVEC;
Tripterygium Glycosides Tablets;
collagen-induced arthritis;
synovial angiogenesis
- MeSH:
Angiogenesis Inhibitors;
pharmacology;
Angiotensin I;
metabolism;
Animals;
Arthritis, Experimental;
chemically induced;
drug therapy;
Drugs, Chinese Herbal;
pharmacology;
Glycosides;
pharmacology;
Human Umbilical Vein Endothelial Cells;
drug effects;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Synovial Membrane;
drug effects;
Tablets;
Tripterygium;
chemistry;
Vascular Endothelial Growth Factor A
- From:
China Journal of Chinese Materia Medica
2019;44(16):3441-3447
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of Tripterygium Glycosides Tablets on angiogenesis of rats with type Ⅱ collagen-induced arthritis( CIA) and on the tube formation of human umbilical vein endothelial cells( HUVEC) in vitro. The HUVEC were induced by 20 μg·L-1 vascular endothelial growth factor( VEGF) in vitro,and were treated with 0. 1,1,10 mg·L-1 Tripterygium Glycosides Tablets continuously for 7 hours. The numbers of branches of tube formation were measured. SD rats were immunized to establish CIA. CIA rats were treated with 9,18,36 mg·kg-1·d-1 Tripterygium Glycosides Tablets for 42 days. Histopathological examination( HE) was performed to observe the vascular morphology and vascular density in the synovial membrane of the inflamed joints. Immunohistochemistry and immunofluorescence were performed to observe the expression of platelets-endothelial cell adhesion molecule( CD31) and αsmooth muscle actin( αSMA) in synovial membrane. Immunohistochemistry and Western blot were performed to observe the expression of hypoxia-inducible factors 1α( HIF1α) and angiotensin 1( Ang1) in the synovial tissue. The results showed that the numbers of branches of tube formation of HUVEC induced by VEGF were improved,and declined significantly after treated by Tripterygium Glycosides Tablets. Compared with the normal group,the vascular density,CD31 positive expression,CD31 +/αSMA-immature and total vascular positive expression in the synovial membrane of the model group were significantly increased,and so as HIF1α and Ang1 in the synovium. Tripterygium Glycosides Tablets reduced the synovial vascular density and inhibited the positive expression of CD31,CD31+/αSMA-immature blood vessels and total vascular,but has no effect on CD31+/αSMA+mature blood vessels. Tripterygium Glycosides Tablets also inhibited the expression of HIF1α and Ang1 in synovial membrane of inflammatory joints. Our results demonstrated that Tripterygium Glycosides Tablets could inhibit the angiogenesis of synovial tissue in CIA rats and the tube formation of HUVEC,which is related to the down-regulation of HIF1α/Ang1 signal axis.
