Therapeutic window analysis of Tripterygium wilfordii processed by liquorice: an instantiation using allergic contact dermatitis.
10.19540/j.cnki.cjcmm.20190710.402
- Author:
Xiao-Hong YU
1
;
Jie-Ming DONG
1
;
Hao WU
1
;
Jia-Bo WANG
2
;
Zhi-Jie MA
1
;
Cong-En ZHANG
1
Author Information
1. Beijing Friendship Hospital,Capital Medical University Beijing 100050,China.
2. the Fifth Medical Center of PLA General Hospital,China Military Institure of Chinese Medicine Beijing 100039,China.
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii processed by liquorice;
allergic contact dermatitis model;
relationship of dose-toxicity-effect;
therapeutic window
- MeSH:
Animals;
Cytokines;
blood;
Dermatitis, Allergic Contact;
drug therapy;
Drugs, Chinese Herbal;
pharmacology;
Glycyrrhiza;
chemistry;
Plant Extracts;
pharmacology;
Tripterygium;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(16):3454-3459
- CountryChina
- Language:Chinese
-
Abstract:
The present study was aimed to explore the dose-toxicity-effect relationship of Tripterygium wilfordii Hook f( TW) processed by liquorice,to establish the safe and effective therapeutic window,and further to provide scientific reference for the clinical use of TW. The toxicity and anti-inflammatory effect of six doses of raw TW and TW processed by liquorice( 0. 78,1. 56,3. 12,6. 24,12. 48,15. 60 g·kg-1) in 1-fluoro-2,4-dinitrobenzene( DNFB)-induced allergic contact dermatitis( ACD) model were mainly examined by histopathology and serum biochemistry. The liver biochemical parameters including ALT and AST,related inflammatory factors including TNF-α and IL-2,together with liver index,kidney index and the other pharmacodynamic indicators,were examined and compared. The results showed that compared with the control group,the serum levels of TNF-α and IL-2 of the model group were significantly increased( P<0. 01),which proved that the ACD model was successful. The comprehensive analysis of liver biochemical indexes,serum inflammatory factors and the other indexes showed that the safe and effective therapeutic window of TW processed by liquorice was 3. 12-12. 48 g·kg-1. The results showed the therapeutic window of TW processed by liquorice was much broader than that of raw TW. And it could provide scientific reference for the clinical rational use of TW.
