Bioinformatics and tissue distribution analysis of Tripterygium wilfordii CYP450.
10.19540/j.cnki.cjcmm.20190603.401
- Author:
Jie GAO
1
;
Yi-Feng ZHANG
2
;
Jia-Wei ZHOU
2
;
Xiao-Yi WU
1
;
Wei GAO
3
;
Lu-Qi HUANG
4
Author Information
1. School of Traditional Chinese Medicine,Capital Medical University Beijing 100069,China.
2. School of Traditional Chinese Medicine,Capital Medical University Beijing 100069,China School of Pharmaceutical Sciences,Capital Medical University Beijing 100069,China.
3. School of Traditional Chinese Medicine,Capital Medical University Beijing 100069,China School of Pharmaceutical Sciences,Capital Medical University Beijing 100069,China Advanced Innovation Center for Human Brain Protection,Capital Medical University Beijing 100069,China.
4. State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
CYP450;
Tripterygium wilfordii;
bioinformatics
- MeSH:
Computational Biology;
Cytochrome P-450 Enzyme System;
chemistry;
Phylogeny;
Plant Proteins;
chemistry;
Proteomics;
Tissue Distribution;
Tripterygium;
enzymology
- From:
China Journal of Chinese Materia Medica
2019;44(16):3594-3600
- CountryChina
- Language:Chinese
-
Abstract:
Cytochrome P450 family is a kind of biocatalyst widely existing in nature. It has many functions such as catalyzing the biosynthesis of plant secondary metabolites and regulating phytoremediation. Based on the analysis of proteome data of Tripterygium wilfordii,the CYP450 gene of T. wilfordii was preliminarily analyzed and predicted by various bioinformatics methods. The results showed that after the expression of T. wilfordii suspension cells was induced by methyl jasmonate,the proteomic data of T. wilfordii were obtained and analyzed,and 10 CYP450 proteins of T. wilfordii were finally screened out. By analyzing the phylogenetic tree constructed with CYP450 gene of Arabidopsis family,the 10 CYP450 proteins were clustered into 6 different CYP450 families. The physical and chemical properties of CYP450 proteins in different families were different. The secondary structure of CYP450 proteins was mainly composed of irregular curls. Eight subcellular localization results of CYP450 proteins were chloroplasts and the rest were plastids. Subsequently,the conserved domains( heme active sites) shared by CYP450 genes were found by analyzing the results of multiple sequence alignment. Finally,by analyzing the transcriptome data of T. wilfordii,the expression distribution of T. wilfordii in different tissues was preliminarily confirmed,which verified its correlation with the biosynthesis of active components of T. wilfordii,and provided important genetic resources for the analysis of biosynthesis pathway of active components of T. wilfordii.
