Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas.
10.19540/j.cnki.cjcmm.20190701.103
- Author:
Rui-Qi SONG
1
;
Tie-Gui NAN
2
;
Yuan YUAN
3
;
Yan JIN
2
;
Quan YANG
1
;
Min ZHANG
4
;
Kai-Yong HU
4
Author Information
1. School of Traditional Chinese Medicine,Guangdong Pharmaceutical University Guangzhou 510006,China.
2. State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
3. School of Traditional Chinese Medicine,Guangdong Pharmaceutical University Guangzhou 510006,China State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
4. Monyan Pharma Jingmen 448124,China.
- Publication Type:Journal Article
- Keywords:
Polyporus umbellatus polysaccharide;
UPLC-MS/MS;
monosaccharide composition;
pre-column derivation
- MeSH:
China;
Chromatography, High Pressure Liquid;
Geography;
Monosaccharides;
chemistry;
Polyporus;
chemistry;
Polysaccharides;
chemistry;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(17):3608-3614
- CountryChina
- Language:Chinese
-
Abstract:
In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.
