Analysis of genomic SSRs in Pueraria lobata and P. thomsonii and establishment of DNA identity card for different germplasms of P. thomsonii of Jiangxi province.
10.19540/j.cnki.cjcmm.20190527.106
- Author:
Rong-Rong ZHOU
1
;
Jun-Hui ZHOU
2
;
Tie-Gui NAN
2
;
Chao JIANG
2
;
Hai-Yan DUAN
2
;
Yu-Ping ZHAO
2
;
Lu-Qi HUANG
1
;
Yuan YUAN
2
Author Information
1. School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
DNA identity card;
Pueraria lobata;
Pueraria thomsonii;
genomic SSR
- MeSH:
China;
DNA, Plant;
genetics;
Genomics;
Microsatellite Repeats;
Polymorphism, Genetic;
Pueraria;
genetics
- From:
China Journal of Chinese Materia Medica
2019;44(17):3615-3621
- CountryChina
- Language:Chinese
-
Abstract:
The type and frequency of simple sequence repeats( SSRs) in the genomes was investigated using the DNA sequence data of Pueraria lobata and P. thomsonii. Based on these SSRs,20 pairs of SSR primers were designed and 5 high polymorphism primer pairs were selected to analyze genetic diversity of 9 cultivars of P. thomsonii in Jiangxi province. The results showed that the 5 pairs of primers could generate 16 polymorphic alleles bands. The average polymorphism information content( PIC) of each SSR primer pair was 0. 600 7.According to the genetic similarity coefficients,the 9 cultivars of P. thomsonii can be classified into 6 germplasms. This study established DNA identity cards with 5 pairs of SSR primers for different germplasm resources of P. thomsonii in Jiangxi province,which provided reference information for the selection of fine germplasms of P. thomsonii and the theoretical basis for the study of Dao-di herbs.
