Grade evaluation of Gardeniae Fructus based on quality constant method.
10.19540/j.cnki.cjcmm.20190629.309
- Author:
Jia SHI
1
;
Wen-Guang JING
2
;
Zhe DENG
2
;
Li SHEN
3
;
Xue-Ting ZHANG
1
;
Jing LIU
1
;
Cong GUO
1
;
Chang CHEN
1
;
An LIU
1
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Traditional Chinese Medicine Institute of China National Traditional Chinese Medicine Corporation Beijing 102600,China.
3. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China.
- Publication Type:Journal Article
- Keywords:
Gardeniae Fructus;
grades evaluation;
quality constant
- MeSH:
Animals;
Drugs, Chinese Herbal;
analysis;
standards;
Fruit;
chemistry;
Gardenia;
chemistry;
Mice;
Quality Control;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2019;44(17):3732-3737
- CountryChina
- Language:Chinese
-
Abstract:
Grade evaluation method of quality constant is a kind of grading method for Chinese medicinal materials and decoction pieces,combining the external morphological index and internal content index. This method was used in this paper for grade evaluation of Gardeniae Fructus. By measuring the morphological and content indexes of 14 batches of Gardeniae Fructus,a method for calculating the quality constant of fruits was established,and the grade evaluation criteria were formed. At the same time,the NO inhibition effect of different grades of Gardeniae Fructus samples on RAW264. 7 cells induced by LPS was determined to investigate the relationship between the quality grade and pharmacodynamics of decoction pieces. The results showed that the quality constants of Gardeniae Fructus decoction piece samples ranged from 1. 46 to 4. 42. If the percentage quality constant ≥80% was classified into first-class,50%-80%as second-class and the rest as third-class,the quality constant was ≥3. 54 for first-class,2. 21-3. 54 for second-class and <2. 21 for third-class Gardeniae Fructus decoction pieces. The pharmacodynamic results showed that the pharmacodynamic intensity was positively correlated with the grade,which also proved the rationality of the grade evaluation method of quality constant.
