Study on chemical constituents of stems and leaves of Sapium discolor.
10.19540/j.cnki.cjcmm.20190614.201
- Author:
Yong-Li ZHANG
1
;
Qi-Ming PAN
2
;
Gui-Jie ZHANG
1
;
Dong LIANG
2
Author Information
1. College of Pharmacy,Guilin Medical University Guilin 541004,China.
2. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources,School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University Guilin 541004,China.
- Publication Type:Journal Article
- Keywords:
Sapium discolor;
anti-neuroinflammatory;
chemical constituents;
lignanoids
- MeSH:
Animals;
Cell Line;
Chromatography, High Pressure Liquid;
Mice;
Phytochemicals;
analysis;
Plant Leaves;
chemistry;
Plant Stems;
chemistry;
Sapium;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(17):3738-3744
- CountryChina
- Language:Chinese
-
Abstract:
Seventeen compounds were isolated from the 95% ethanol extract of the stems and leaves of Sapium discolor by using various chromatographic techniques,including silica gel,Sephadex LH-20,MCI,ODS,and semi-preparative HPLC. Their structures were elucidated as sapiumin F( 1),kadsulignan C( 2),ciwujiatone( 3),ethylbrevifolin carboxylate( 4),7-hydroxy-8-methoxycoumarin( 5),fraxetin( 6),fraxidin( 7),isofraxidin( 8),6,7,8-trimethoxycoumarin( 9),5,6,7,8-tetramethoxycoumarin( 10),8-hydroxy-5,6,7-trimethoxycoumarin( 11),3,3'-di-O-methylellagic acid( 12),3,3',4'-tri-O-methylellagic acid( 13),3'-methoxyellagic acid 4'-O-α-rhamnopyranoside( 14),4,5-didehydro-chebulic acid triethyl ester( 15),ent-kaurane-3-oxo-16α,17-diol( 16),and abscisic acid( 17) by spectroscopic data. Compound 1 is a new compound. Except for compounds 4,11,and 13,the remaining compounds were isolated from this plant for the first time. All the isolates were evaluated for their in vitro antineuroinflammatory activities,and the results showed that compounds 6 and 15 significantly inhibited nitric oxide production in lipopolysaccharide-induced BV-2 microglial cells,with IC50 values of 6. 06 and 6. 05 μmol·L-1,respectively.
