Mechanism of Calculus Bovis Sativus in inhibiting hepatocyte lipid deposition based on serum pharmacology.
10.19540/j.cnki.cjcmm.20190416.402
- Author:
Wen-Xi HE
1
;
Cheng-Liang ZHANG
1
;
Dong XIANG
1
;
Jin-Yu YANG
1
;
Yan-Jiao XU
1
;
Xiu-Hua REN
1
;
Dong LIU
1
Author Information
1. Department of Pharmacy of Tongji Hospital,Tongji Medical School,Huazhong Science and Technology University Wuhan 430030,China.
- Publication Type:Journal Article
- Keywords:
Calculus Bovis Sativus;
fatty degeneration;
fructose;
non-alcoholic fatty liver disease;
serum pharmacology
- MeSH:
Animals;
Apoptosis;
Cattle;
Cells, Cultured;
Fatty Liver;
Fructose;
Gallstones;
chemistry;
Hepatocytes;
cytology;
metabolism;
Lipid Metabolism;
Lipid Peroxidation;
Liver;
Medicine, Chinese Traditional;
Mice;
Reactive Oxygen Species;
metabolism;
Serum;
chemistry;
Sterol Regulatory Element Binding Protein 1;
metabolism;
Triglycerides
- From:
China Journal of Chinese Materia Medica
2019;44(17):3780-3785
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the molecular mechanism of Calculus Bovis Sativus( CBS) in alleviating lipid accumulation in vitro by serum pharmacology. The CBS-containing serum of mice was obtained by serum pharmacology method to evaluate its effect on the proliferation of LO2 hepatocytes. The lipid reducing effects of CBS-containing serum through Nrf2 was evaluated by fructose-induced LO2 hepatocyte steatosis model,nuclear factor erythroid 2 related factor 2( Nrf2) agonist oltipraz combined intervention,cell oil red O staining and intracellular triglyceride( TG) content. The effects of CBS-containing serum on lipid peroxidation and hepatocytes apoptosis were evaluated by reactive oxygen species( ROS) and apoptosis assay,respectively. Real-time quantitative polymerase chain reaction( PCR) was used to detect the relative expression of lipid synthesis-related genes and apoptosis-related genes.RESULTS:: showed that CBS drug-containing serum had no significant effect on LO2 hepatocyte proliferation. As compared with the model group,CBS-containing serum could effectively reduce the formation of lipid droplets in fructose-induced LO2 hepatocytes,significantly reduce intracellular TG and ROS levels,and significantly reduce hepatocyte apoptosis rate( P < 0. 05). As compared with the model group,carbohydrate responsive element binding protein( ChREBP),sterol regulatory element binding protein-1 c( SREBP-1 c),fatty acid synthase( FAS),acetyl-CoA carboxylase 1( ACC1),stearoyl-CoA desaturase 1( SCD1),Bax and caspase-3 mRNA levels were significantly reduced in CBS drug-containing serum treatment group( P<0. 05). All of the above effects could be reversed by oltipraz.In conclusion,CBS-containing serum can significantly inhibit the fructose-induced LO2 liver fat deposition,and the mechanism may be related to reducing intracellular ROS level through the Nrf2 pathway and improving intracellular peroxidation state to reduce apoptosis.
