Acute toxicity mechanism of Panax notoginseng saponins in larvae zebrafish based on metabonomics.
10.19540/j.cnki.cjcmm.20190429.503
- Author:
Qian-Qian FEI
1
;
Ying-Jie WEI
1
;
Jing WANG
1
;
Yi-Ping HUANG
1
;
Yan CHEN
1
;
Bin CHEN
1
Author Information
1. the Third Clinical Medical College (Affiliated Provincial Hospital of Integrated Traditional Chinese Medicine and Western Medicine) ,Nanjing University of Chinese Medicine,Key Laboratory for Traditional Chinese Medicine Drug Release System under State Administration of Traditional Chinese Medicine,Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028,China.
- Publication Type:Journal Article
- Keywords:
Panax notoginseng saponins;
acute toxicity;
larvae zebrafish;
metabonomics
- MeSH:
Amino Acids;
metabolism;
Animals;
Chromatography, High Pressure Liquid;
Energy Metabolism;
Larva;
drug effects;
Lipid Metabolism;
Mass Spectrometry;
Metabolomics;
Panax notoginseng;
toxicity;
Saponins;
toxicity;
Toxicity Tests, Acute;
Zebrafish
- From:
China Journal of Chinese Materia Medica
2019;44(17):3798-3805
- CountryChina
- Language:Chinese
-
Abstract:
Based on metabolomics,the metabolites of larvae zebrafish with overdose of Panax notoginseng saponins( PNS) were compared with those in normal group of larvae zebrafish to investigate the possible toxicity mechanism of overdose PNS in larvae zebrafish. An experimental animal model of long-term toxicity induced by PNS overdose was established by administering 1-6 dpf at low,medium and high doses of PNS,respectively. The ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry( UPLC-Q-TOF-MS) technique was combined with principal component analysis( PCA) and orthogonal partial least squares discriminant analysis( OPLS-DA) to screen and identify biomarkers associated with toxicity,and then the MetaboAnalyst database was used to analyze metabolism-related pathways. The results showed that the metabolites of each group could be distinguished distinctly,and they deviated more from the normal group in a time and dose dependent manner. Twenty-nine potential biomarkers related to toxicity( VIP>1,P<0. 05) were identified preliminarily,mainly involving six metabolic pathways. From the metabonomics point of view,the toxicity mechanism of overdose PNS may be related to the disorders of lipid metabolism,amino acid metabolism and energy metabolism.
