rhPDCD5 suppresses pro-inflammatory cytokine secretion and proliferation and induces apoptosis of activated lymphocytes from rats with collagen-induced arthritis.
10.12122/j.issn.1673-4254.2019.06.01
- Author:
Wang WANLIN
1
;
Mao CHUN
2
;
Xiao JUAN
2
Author Information
1. Zaoyang First People's Hospital, Hubei University of Arts and Science, Xiangyang 441200, China.
2. Laboratory of Molecular Medicine, Hubei University of Arts and Science, Xiangyang 441053, China.
- Publication Type:Journal Article
- Keywords:
activation-induced cell death;
lymphocytes;
recombinant human PDCD5;
rheumatoid arthritis
- MeSH:
Animals;
Apoptosis;
Apoptosis Regulatory Proteins;
Arthritis, Experimental;
Cattle;
Cell Proliferation;
Cytokines;
Female;
Humans;
Lymphocytes;
Neoplasm Proteins;
Rats;
Rats, Wistar
- From:
Journal of Southern Medical University
2019;39(6):627-632
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of recombinant human PDCD5 (rhPDCD5) treatment in a rat model of bovine II collagen (CII)-induced arthritis (CIA) on inflammatory cytokine secretion, proliferation and apoptosis of activated lymphocytes and explore the mechanisms of rhPDCD5-induced immunosuppression on activated lymphocytes.
METHODS:Female Wistar rats were randomly divided into normal control group, CIA+ ovalbumin (OVA) group, CIA+ rhTNFR: Fc group, and CIA+rhPDCD5 group. The rats in the latter 3 groups received intraperitoneal injections of OVA (14 mg/kg), rhTNFR: Fc (3.5 mg/kg) or rhPDCD5 (14 mg/kg) from day 2 to day 26 following CII injection. On day 28, the spleens of the rats were harvested for preparing single cell suspensions of splenocytes, which were activated by CII (20μg/mL) or anti-CD3 (1μg/mL)+ anti-CD28 (2μg/mL) for 48 h and 72 h. The production of interferon-γ(IFN-γ) and interleukin-17A (IL-17A) by the activated lymphocytes was determined by ELISA of the culture supernatants. The proliferation and apoptosis of the activated lymphocytes were assessed using [H]-thymidine incorporation assay and flow cytometry, respectively.
RESULTS:Compared with those in CIA + OVA group, IFN-γand IL-17A secretions by the activated lymphocytes from rhPDCD5-treated CIA rats significantly decreased. RhPDCD5 treatment of the CIA rats obviously suppressed the proliferation and promoted apoptosis of the lymphocytes activated by CII or by anti-CD3 + anti-CD28.
CONCLUSIONS:rhPDCD5 reduces pro-inflammatory cytokine secretion, inhibits the proliferation and promotes activation-induced cell death of activated CD4 lymphocytes to produce immunosuppression in rat models of CIA.
