Medium-chain acyl-CoA dehydrogenase enhances invasion and metastasis ability of breast cancer cells.
10.12122/j.issn.1673-4254.2019.06.04
- Author:
Yinjue YU
1
;
Linfeng ZHAO
1
;
Rong LI
1
Author Information
1. Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
breast cancer;
invasion;
medium chain acyl-CoA dehydrogenase;
migration
- MeSH:
Acyl-CoA Dehydrogenase;
Animals;
Breast Neoplasms;
Cell Movement;
Cell Proliferation;
Epithelial-Mesenchymal Transition;
Humans;
MCF-7 Cells;
Mice
- From:
Journal of Southern Medical University
2019;39(6):650-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of medium-chain acyl-CoA dehydrogenase (ACADM) on invasion and metastasis of breast cancer cells and explore the underlying mechanism.
METHODS:A large cancer genome database was used to analyze the expression of ACADM in breast cancer tissues and normal tissues. The proliferation, migration and invasion of cultured breast cancer MCF-7 and T47D cells with ACADM overexpression or ACADM silencing were evaluated using MTT proliferation assay, EdU assay, Transwell chamber assay, and Boyden invasion assay; Western blotting was used to detect the protein expressions of the related pathway in the cells. In nude mouse models of tail vein metastasis of MCF-7 cells with or without ACADM overexpression, the tumor growth and tumor histopathology were observed using HE staining.
RESULTS:Analysis of the Oncomine sample set showed a significantly higher expression level of ACADM in breast cancer tissues than in normal breast tissues ( < 0.05). Overexpression of ACADM obviously enhanced the migration and invasion abilities and promoted the epithelial-mesenchymal transition (EMT) of cultured MCF-7 and T47D cells; conversely, silencing of ACADM significantly suppressed the migration and invasion of the breast cancer cells. In the nude mouse models, ACADM overexpression in MCF-7 cells significantly enhanced their migration and invasion abilities.
CONCLUSIONS:ACADM can promote the EMT process of breast cancer cells and improve the migration and invasion ability. ACADM is an oncogene in breast cancer.
