Plasma miRNA-23a and miRNA-451 as candidate biomarkers for early diagnosis of nonsmall cell lung cancer: a case-control study.
10.12122/j.issn.1673-4254.2019.06.12
- Author:
Shengjin CUI
1
;
Zhaopeng CAO
1
;
Weiquan GUO
1
;
Huijun YU
1
;
Rong HUANG
1
;
Yunfeng WU
1
;
Yiwen ZHOU
1
Author Information
1. Department of Clinical Laboratory, Shenzhen Hospital of Southern Medical University, Shenzhen 518101, China.
- Publication Type:Journal Article
- Keywords:
Biomarker;
miRNA-23a;
miRNA-451;
non-small cell lung cancer
- MeSH:
Biomarkers, Tumor;
Carcinoma, Non-Small-Cell Lung;
genetics;
Case-Control Studies;
Early Detection of Cancer;
Humans;
Intracellular Signaling Peptides and Proteins;
Lung Neoplasms;
genetics;
Membrane Proteins;
MicroRNAs;
ROC Curve
- From:
Journal of Southern Medical University
2019;39(6):705-711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the value of plasma miRNA23-a and miRNA-451 as potential biomarkers for early diagnosis of non-small cell lung cancer (NSCLC).
METHODS:Fifty patients with NSCLC and 50 healthy control subjects were recruited for testing the plasma levels of miRNA23-a and miRNA-451 and their expression levels in the tumor tissues using qRT-PCR. The correlations of the plasma levels of miRNA23-a and miRNA-451 with their expressions in the tumor tissues were analyzed. The diagnostic power of CEA, miRNA23-a and miRNA-451 for NSCLC was evaluated using the receiver-operating characteristics (ROC) curves and the area under the ROC curves (AUC). In the NSCLC cell line A549, we tested the effect of inhibition of miRNA-23a and miRNA-451 on the expression levels of SPRY2 and MIF mRNA using qRT-PCR.
RESULTS:The expression levels of miRNA-23a and miRNA-451 in NSCLC tissues was significantly associated with smoking, tumor size, lymph node metastasis and TNM stage ( < 0.05). Compared with those in the control group, miRNA-23a level was significantly increased while miRNA-451 was significantly down-regulated in the tumor tissues and plasma of NSCLC patients. The plasma levels of miRNA-23a and miRNA-45 were strongly correlated with their expression levels in the tumor tissues. ROC analysis showed that for the diagnosis of NSCLC, the AUC, sensitivity and specificity of either miRNA-23a or miRNA-451 were significantly higher than those of CEA ( < 0.05). The combination of miRNA23-a and miRNA-451 markedly improved the AUC (0.900), sensitivity (78%) and specificity (86%) for the diagnosis. In A549 cells, inhibition of miRNA23-a and miRNA-451 resulted in significantly increased expression levels of SPRY2 mRNA and MIF mRNA, respectively.
CONCLUSIONS:miRNA-23a and miRNA-451 can be used as potential biomarkers for early diagnosis of NSCLC, and their combined detection can be more effective for the diagnosis.
