Correlation between methylation level of and genes and aging in healthy individuals.
10.12122/j.issn.1673-4254.2019.06.15
- Author:
Zhonghua ZHENG
1
;
Huihui JI
1
;
Chujia CHEN
1
;
Yin LI
1
;
Shiwei DUAN
1
Author Information
1. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315211, China.
- Publication Type:Journal Article
- Keywords:
CDKN2A;
CDKN2B;
DNA methylation;
aging
- MeSH:
Aging;
Cyclin-Dependent Kinase Inhibitor p15;
metabolism;
Cyclin-Dependent Kinase Inhibitor p16;
metabolism;
DNA Methylation;
Female;
Humans;
Male;
Real-Time Polymerase Chain Reaction
- From:
Journal of Southern Medical University
2019;39(6):724-730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the relationship between and gene methylation with aging in the general population.
METHODS:We collected peripheral blood samples from 284 male and 246 female healthy subjects for detection of methylation levels of and genes using quantitative methylation-specific PCR (qMSP). The relationship between the methylation levels of and genes and aging was analyzed using Spearman or Pearson correlation test.
RESULTS:We found a significant positive correlation between the methylation levels of the two genes in these subjects ( < 0.05). In the overall population as well in the female subjects, methylation was found to be inversely correlated with age ( < 0.05). The methylation levels of and genes were inversely correlated with TG, ApoE, Lp(a) and AST in the overall population ( < 0.05). In both the female and male subjects, the methylation levels of the two genes were inversely correlated with Lp(a) ( < 0.05). In the male subjects, methylation was inversely correlated with AST ( < 0.05), while methylation was inversely correlated with HDL and ApoE ( < 0.05). In the female subjects, methylation was positively correlated with LDL and inversely correlated with ApoE and AST ( < 0.05).
CONCLUSIONS:The methylation levels of and are closely related to age and the levels of multiple proteins in healthy subjects.
