Overexpression of histone deacetylase 11 suppresses basal-like breast cancer cell invasion and metastasis.
10.12122/j.issn.1673-4254.2019.07.01
- Author:
Zhang YI
1
;
Luo WENWEN
1
;
Wang KUN
2
;
Shi JIAN
1
Author Information
1. Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
2. Second Department of Breast Cancer, Cancer Center, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
- Publication Type:Journal Article
- Keywords:
Histone deacetylase 11;
Twist;
basal-like breast cancer;
invasion;
metastasis
- MeSH:
Animals;
Breast Neoplasms;
Histone Deacetylases;
Mice;
Mice, Nude;
Neoplasm Invasiveness;
Neoplasm Metastasis;
Promoter Regions, Genetic;
Twist-Related Protein 1
- From:
Journal of Southern Medical University
2019;39(7):751-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Histone deacetylase 11 (HDAC11) is a class Ⅳ member of histone deacetylase family, and its role in regulating cancer cell invasion and metastasis remains unclear. We aimed to investigate the role of HDAC11 in regulating the biological behaviors of basal-like breast cancer (BLBC) cells.
METHODS:We analyzed the expression of HDAC11 based on Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA). The effects of HDAC11 on the cell invasion and metastasis were examined using Transwell assay and in a mouse model. The interaction between HDAC11 and Twist was detected with immunoprecipitation. We identified 2 as a target gene of Twist using promoter luciferase assay and chromatin immunoprecipitation assay.
RESULTS:HDAC11 was lowly expressed in BLBC cells. HDAC11 overexpression suppressed BLBC cell invasion and their metastasis in nude mice. Mechanistically, HDAC11 directly interacted with Twist protein, antagonized its pro-invasive function and repressed Twist-induced 2 gene transcription.
CONCLUSIONS:Our data suggest that HDAC11 acts as a negative modulator of invasion and metastasis of BLBC cells.
