Role of miRNA-340 in modulating gastric cancer cell proliferation and bioinformatic analysis.
10.12122/j.issn.1673-4254.2019.07.06
- Author:
Jian WANG
1
;
Wenjing CHEN
1
;
Huijuan LIN
1
;
Jiangyu ZHANG
1
Author Information
1. Department of Pathology, Guangdong Women and Children's Hospital Affiliated to Guangzhou Medical University, Guangzhou 510000, China.
- Publication Type:Journal Article
- Keywords:
bioinformatics;
circular RNA;
gastric cancer;
miRNA-340;
signal pathways
- MeSH:
Animals;
Cell Line, Tumor;
Cell Proliferation;
Computational Biology;
Gene Expression Regulation, Neoplastic;
Mice;
MicroRNAs;
Stomach Neoplasms
- From:
Journal of Southern Medical University
2019;39(7):784-790
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism of miRNA-340 for regulating the proliferation of gastric cancer (GC) cells and predict its interacting circular RNAs (circRNAs), its downstream target genes and the involved signaling pathways.
METHODS:The differentially expressed miRNAs in GC cell lines were analyzed and screened using miRNA microarrays. The expression level of miRNA-340 in 21 pairs of GC tissues and adjacent normal tissues was detected using real-time PCR. MTT and EdU assays were performed to examine the effect of miRNA-340 on the proliferation ability of HFE145 and BGC-823 cells. We also tested the effect of miRNA-340 inhibition on subcutaneous tumorigenesis of GC cells in a nude mouse model. The downstream target genes of miRNA-340 and the probable signal pathways were predicted online using Targetscan and DAVID database, respectively. The interacting circRNAs of miRNA-340 were analyzed using starBase platform.
RESULTS:Among the differentially expressed miRNAs, miRNA-340 was significantly down-regulated in GC cell lines. Real-time PCR results showed that the expression of miRNA-340 was significantly lower in GC tissues than in the adjacent tissues ( < 0.05). MTT and EdU cell proliferation assays showed that miRNA-340 overexpression inhibited the proliferation of GC cells in vitro. In the nude mouse models, the proliferation of GC cells transfected with miRNA-340 inhibitor was obviously enhanced. Bioinformatics analysis suggested that miRNA-340 had 21 target genes with 3 or more conserved sites, and these genes were involved in tumorigenesis and invasion. The top 10 circRNAs were selected as the most powerful sponge circRNAs interacting with miRNA-340.
CONCLUSIONS:miRNA-340 may play the role of a tumor suppressor in tumorigenesis and progression. Overexpression of miRNA-340 suppress the proliferation of GC cells, suggesting its involvement in the development of GC along with multiple circRNAs.
