Design, screening and antimicrobial activity of novel peptides against .
10.12122/j.issn.1673-4254.2019.07.12
- Author:
Dongsheng LIANG
1
;
Huanying LI
1
;
Xiaohu XU
2
;
Jingheng LIANG
1
;
Xingzhu DAI
1
;
Wanghong ZHAO
1
Author Information
1. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
2. Department of Stomatology, Shenzhen Longhua District Central Hospital, Shenzhen 518110, China.
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
antimicrobial activity;
antimicrobial peptides;
dental caries;
imperfect amphipathicity
- MeSH:
Anti-Infective Agents;
Dental Caries;
Humans;
Microbial Sensitivity Tests;
Peptides;
Streptococcus mutans
- From:
Journal of Southern Medical University
2019;39(7):823-829
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct antimicrobial peptides with potent antimicrobial activity, low cytotoxicity and efficient killing rate of for prevention and treatment of dental caries.
METHODS:We exploited the existing design strategies to modify reutericin 6 or gassericin A produced by species in the oral cavity based on their cationicity, amphipathicity and -helical structure. We examined their antimicrobial activities using bacterial susceptibility assay, their cytotoxicity through cytotoxicity assay and their killing rate of with time-kill assay. We further evaluated the candidate derivatives for their killing rate against , their antimicrobial activity against different oral pathogens and the development of drug resistance.
RESULTS:We constructed 6 AT-1 derivatives, among which AT-7 showed an MIC of 3.3 μmol/L against , and with a killing rate of 88.7% against within 5 min. We did not obtain strains of resistant to AT- 7 after induction for 10 passages.
CONCLUSIONS:Hydrophobicity and imperfect amphipathic structure are two key parameters that define the antimicrobial potency of the antimicrobial peptides. The imperfectly amphipathic peptide AT-7 shows the potential for clinical application in dental caries treatment.
